Other

The City University of New York

Yang Pu

Robert R Alfano

Wubao Wang

Lingyan Shi

Laura A Sordillo

Yury Budansky

Guichen C Tang

Sebastião Pratavieira

James Eastham

A method is provided for deep tissue imaging using multi-photon excitation of a fluorescent agent. The fluorescent agent is irradiated with an ultrafast laser to produce an excited singlet state (S) which subsequently undergoes non-radiative relaxation to a first singlet state (S). The Sstate undergoes fluorescence to the ground state Sto produce an emission wavelength. Both the excitation and emission wavelengths are within the near infrared optical window, thereby permitting deep tissue penetration for both the incoming and outgoing signals.

Method of deep tissue imaging using multi-photon excitation of a fluorophore

A rectal near infrared (NIR) scanning polarization imaging system uses NIR Photonic Prostatoscopy Analyzer (NIRPPA) for prostate cancer detection using light. The NIRPPA consists of a portable rectal NIR scanning polarization imaging unit and an optical fiber-based rectal probe capable of recording sets of 2D images of a prostate through rectum at different wavelengths and depths and obtaining a three dimensional (3D) image of the prostate and 3D locations of abnormal tissue inside the prostate. Diode lasers/light emission diodes (LEDs) with selected emitting wavelengths are used in the NIR spectral range from 650 nm to 2,400 nm corresponding to the four tissue optical windows (#I, 650 nm-950 nm; #II, 1,100 nm-1,350 nm; #III, 1,600 nm-1,870 nm; and #IV, 2,100 nm-2,300 nm). The fingerprint absorptions of water (HO), Oxyhemoglobin (HbO) and deoxyhemoglobin (Hb) in the prostate are used as native biomarkers for prostate cancer detection.

Near infrared photonic prostatoscopy analyzer

Coherent light is used to image cells/molecules at wavelengths in the near-infrared (NIR) region in second, third and fourth spectral windows. Optical attenuation from thin tissue slices of normal and malignant breast and prostate tissue, and pig brain are placed between matched bandpass filters, within desired windows and measured within an NIR spectral window at wavelengths selected to highlight the desired cells/molecules. Due to a reduction in scattering and minimal absorption, longer attenuation and clearer images can be seen in the second, third and fourth NIR windows compared to the conventional first NIR window. The spectral windows have uses in microscope imaging one or more collagens, elastins, lipids and carotenoids in arteries, bones, breast, cells, skin, intestines, bones, cracks, teeth, and blood due to less scattering of light and improved signal to noise to provide clearer images.

Deep optical imaging of tissue with less scattering in the second, third and fourth NIR spectral windows using supercontinuum and other laser coherent light sources

Spatial frequency spectra from periodic, aperiodic and quasi-random structures in materials are shown and used to detect differences among objects via internal coding from the spatial frequencies. The method is applied to different grades of human tissues for a new form of histology and pathology, and to detect art forgeries and coding boxes, money and papers and gems. The randomness of material structures on surface and at depths near surface can be detected from the spatial spectrum. In tissue spectral features from normal to different stages of cancer in tissue for ex vivo and in vivo applications can be recognized by different spectral fingerprints content of the spatial frequency. Similarly, the painting for the strokes of artist is different. A new type of instrument is described to analyze materials as a Spatial Frequency Spectrometer.

Spatial frequency spectrometer for and method of detection of spatial structures in materials

A method detects the degree of spoilage of food by exposing a food sample to an excitation wave having a first wavelength of about 340 nm or about 380 nm, wherein the excitation wave has a bandwidth of 40 nm or less. The excitation wave is permitted to interact with the food sample and return emission spectra. A detector detects the emission spectra. A predetermined threshold value is established which defines when a food sample is or is not spoiled. The emission spectra is analyzed at a second wavelength of about 400 nm, about 450 nm or about 530 nm to provide a test or measured value of the emission spectra indicative of the degree of spoilage of the food sample. Whether or not a food sample is spoiled beyond the predetermined threshold is determined by comparing the measured value to the predetermined threshold value.

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New York, NY, United States of America

Yang Pu