Monsanto Technology LLC

Yang Ju Im

Jeffrey Lawrence Kohne

William M Fischer

Matthew J Weis

Wayne Brown

John M Jensen

Michael Joseph Dayawon

David V Butruille

Fenggao Dong

Edward James Cargill

David W Finley

Jonathan C Lamb

Ruth A Wagner

Fengxing Du

Piyaraj Nan Newton

Pierre Sehabiague

Shengqiang Zhong

David Finley

Christopher T Bauer

Daniel Challis

Brian W Gardunia

William Michael Fischer

John Michael Jensen

A seed sampling system is provided comprising an automated seed loading assembly operable to singulate seeds from a plurality of seeds or enable loading of individually stored seeds and an automated seed sampling assembly comprising at least one sampling module operable to remove tissue samples from one of the singulated seeds. The system also includes an automated seed transport assembly comprising at least one retention member operable to transfer the singulated seeds from at least one elevator unit of the seed loading assembly to the at least one sampling module of the seed sampling assembly. In connection therewith, the at least one sampling module includes multiple sampling locations, each associated with a sampler, where the at least one sampling module is operable to remove tissue samples from seeds at one of sampling locations while another one of the sampling locations is cleaned to remove residual seed tissue therefrom.

Automated systems for removing tissue samples from seeds, and related methods

The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

Methods for genotyping haploid embryos

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Ellisville, MO, United States of America

Yang Ju Im