Republic of Korea (National Forensic Service Director Ministry of Public Administration and Security)

Yang-Han Lee

Eu-Ree An

Min Young Lee

Min-Hee Kim

Kyungmyung Lee

Hyun-Chul Park

Ju Yeon Jung

Si-Keun Lim

Ki Won Park

Chong Min Chung

A method is disclosed for analyzing single nucleotide polymorphisms (SNPs) of mitochondrial DNA (mtDNA) using a peptide nucleic acid (PNA) probe and a melting curve analysis, and more particularly, a method is disclosed for analyzing SNPs of mtDNA by the melting curve analysis of a PNA probe and mtDNA, in which the PNA probe is coupled with a reporter and a quencher. By using PNA probes labeled with a reporter and a quencher in the method for analyzing SNPs of mtDNA, the time and cost required for individual identification can be drastically decreased, initial analysis can be carried out in the event of a large number of inspections or mass casualties, and human identification information can be coded, and thus the method is very easy to manage and supervise.

Method for analyzing SNPs of mitochondrial DNA using PNA probes and melting curve analysis

The present disclosure relates to a primer set for preparation of a library used for next generation sequencing (NGS), and a method and a kit for making an NGS library using the same. The method for making the next generation sequencing (NGS) library using the primer set according to the present disclosure is able to simply analyze large amounts of samples as compared to the existing method for making a library, which is effectively usable for analysis of target DNA sequences or for database construction, in large amounts of samples.

Primer set for preparation of NGS library and method and kit for making NGS library using the same

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Gangwon-do, South Korea

Yang-Han Lee