Jiangnan University

Yang Gu

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Jieying Deng

Yang Song

Yawen Zhao

The present invention relates to a method for promoting acetylglucosamine synthesis of, which belongs to the field of genetic engineering. The present invention adopts the recombinantBSGNKAP2 as a starting strain, exogenously introducing pyruvate carboxylase BalpycA derived from, eliminating the central carbon metabolism overflow of theand avoiding the synthesis of the by-product acetoin; further, five exogenous reducing force metabolic reactions are introduced to replace the reaction of generating NADH in glycolysis pathway and tricarboxylic acid cycle to reconstruct intracellular reducing force metabolism, which specifically comprise glyceraldehyde-3-phosphate ferredoxin dehydrogenase, isocitrate NADdehydrogenase, a malate quinone dehydrogenase, a ketoacid ferredoxin oxidoreductase and a nitrogenase ferritin. In a shake-flask fermentation process using a complex medium, acetylglucosamine yield of the recombinant strain BSGNKAP8 is 24.50 g/L, acetylglucosamine/glucose yield is 0.469 g/g, respectively 1.97 times and 2.13 times of those of the starting strain BSGNKAP2.

Method for promoting acetylglucosamine synthesis of bacillus subtilis

The invention relates to a recombinant strain of, wherein pyruvate carboxylase BalpycA, glyceraldehyde-3-phosphate ferredoxin dehydrogenase gor, isocitrate NADdehydrogenase icd, malate quinone dehydrogenase mqo, pyruvate ferredoxin oxidoreductase porAB and nitrogenase ferritin cyh are integrated and expressed in the recombinant strain. The invention also discloses use of the recombinant strain in fermentation production of acetylglucosamine. The recombinantof the invention eliminates the central carbon metabolism overflow of theand balances the intracellular reducing force, and the fermentation yield of acetylglucosamine is greatly improved.

Recombinant strain of

The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineeredDeletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineeredfor GlcNAc production, which can be further applied to industrial production of GlcNAc.

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Wuxi, China

Yang Gu