Jiangnan University

Inner Mongolia Mengniu Dairy (group) Co., Ltd.

Yanfeng Liu

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Xueqin Lv

Rongzhen Tian

Hannes Link

Xiaolong Zhang

Wenyang Wu

Guolin Zhou

Chenyang Zhang

The invention provides astrain for producing a human milk lipid substitute. By integrating a heterologous lysophosphatidic acid acyltransferase intoand knocking out its own natural lysophosphatidic acid acyltransferase, the content of palmitic acid (C16:0) at Sn-2 position of triacylglycerol produced byis increased, to synthesize a human milk lipid substitute. On this basis, a metabolic pathway related gene is knocked out, to further increase the content of human milk lipid substitute in the product. In the present invention, a human milk lipid substitute is de novo synthesized byfor the first time, in which the total fatty acid is 15% or more, and the relative content of C16:0 at Sn-2 position reaches about 60%.

strain for producing human milk lipid substitute and use thereof

The disclosure disclosesfor producing N-acetylneuraminic acid and application thereof, and belongs to the field of genetic engineering. The disclosure optimizes the expression levels of key enzymes in N-acetylneuraminic acid synthesis pathways on genome through promoters of different strength, reduces the protein synthesis pressure caused by the expression of enzymes on cells, and further integrates the three N-acetylneuraminic acids in a sameengineering strain.with improved N-acetylneuraminic acid production is obtained, and the production reaches 10.4 g/L at the shake flask level, laying a foundation for further improving the NeuAc production from

for producing N-acetylneuraminic acid and application thereof

The present disclosure relates to a method for regulating expression of protein of interest in, and belongs to the technical field of genetic engineering. The method comprises: usingas an expression host, adding the N-terminal nucleotide sequence coding the first 15 amino acids of the endogenous protein before the coding gene of the protein of interest or modifying the original N-terminal nucleotide sequence coding the first 15 amino acids, and performing free expression in plasmid, thereby regulating expression of the protein of interest in, and even regulating the expression difference in different growth phases and the expression level.

Method for regulating expression of protein of interest in bacillus subtilis

The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinantwith a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinantwith the glcK knockout were 0.15 hand 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinantof the present invention would be potentially useful for industrial production of GlcNAc.

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Wuxi, China

Yanfeng Liu