Tianjin University

Tasly Pharmaceutical Group Co., Ltd.

Tasly Pharmaceuticals Group Co., Ltd.

Tianjin Tasly Pharmaceutical Co., Ltd.

Yan Sun

Shunnan Zhang

Xiaolin Bai

Jianhui Yang

Qinghong Shi

Lin Zhang

Xijun Yan

Naifeng Wu

Zhengliang Ye

Lihong Zhou

Ting Li

Yan Liu

Lijun Fan

Hui Wang

Xiaoyan Dong

Lina Dong

Yongfeng Zheng

Hongbo Zhang

Jie Ma

Tong Liu

Fufeng Liu

Yanying Li

Xiaodan Liu

Xingyun Shao

Song Gao

Bo Xu

Weiwei Zhao

Guozhen Wang

Hongpo Zhang

An affinity ligand peptide library of IgG constructed on the basis of Protein A affinity model and the application of a design method thereof. According to the Molecular Mechanics&#x2014;Poisson-Boltzmann surface area (MM/PBSA) method and on the basis of the known human IgG-Protein A complex structure, the hot spots of Protein A that have high affinity for human IgG are obtained analytically, and a Protein A simplified affinity model is built thereof. An affinity peptide library of IgG is constructed including heptapeptide and octapeptide structural modes. On the basis of the peptide structural modes, the types of inserted amino acids that 'X' residues represent are further identified using amino acid location method. Then, molecular docking and molecular dynamics simulation methods are used to screen the candidate peptides successively. Finally, the affinity peptide ligands that can effectively purify IgG are identified using affinity chromatography.

Method for constructing a novel affinity peptide library for binding immunoglobulin G based on a protein affinity model of protein A

Affinity peptide ligands of mouse polyomavirus capsomers and a designed screening method thereof. The affinity peptide ligands can be used for separation and purification of the capsomers.

Affinity peptide ligand of mouse polyoma virus capsomer and designed screening method thereof

A pharmaceutical composition for treating headache, prepared from eleven Chinese herbal medicines: Chinese angelica root,, prepared rhizome of rehmannia, uncaria tomentosa, leatherleaf milletia, prunella vulgaris, sicklesenna seed, pearl shell,tuber, and, and a proper amount of adjuvant materials. A preparation method of the pharmaceutical composition, and uses thereof in the preparation of drugs for treating various headaches, traumatic cranial nerve syndrome, dizziness and vertigo, vexation and irritability, insomnia and dreaminess.

Pharmaceutical composition for treating headache, and preparation method thereof

A pharmaceutical granule whose shape is spherical or sphere-like shape with a bulk density of 0.6-1.3 g/ml and a dissolution time of 0.5-5 minutes, which is prepared as follows: mother granules are filled into a fluidized-bed as bed charge; active pharmaceutical ingredients are prepared into a suspension or solution whose viscosity is adjusted to 6.0-9.8 Mpa&#xb7;S with viscosity adjusting agent; then it is sprayed onto surface of said mother granule to obtain final granule.

Granule and preparation method thereof

A method for protein refolding with an ion exchange resin. The method includes (a) choosing an ion exchange resin that having charged groups with the same sign as a net charge of a denatured protein to be refolded; (b) removing heterogeneous ions from the ion exchange resin by washing the ion exchange resin sequentially with saline solution and deionized water, to prepare the ion exchange resin; (c) mixing the ion exchange resin with a refolding buffer thoroughly, then adding the denatured protein to the refolding buffer and allowing the denatured protein to refold; and then (d) collecting the supernatant by centrifugation or settlement, to obtain a solution containing the refolded protein.

Method of protein refolding with ion exchange resins and the application of the same

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Tianjin, China

Yan Sun