Nanjing Bestzyme Bio-engineering Co., Ltd.

Nanjing Genscript Biotech Co., Ltd.

Yan Sun

Feng Li

Hong Xu

Yan Fan

Aixi Bai

Zhenyu Liu

Xiuzhen Du

Jidong Zhu

Ke Huang

Furong Bian

Minghui Hao

Long Fan

Lihua Zhang

Dongming Wu

Xiaoluo Huang

Disclosed herein is a method for expressing phytase in a filamentous fungus by using an optimizedphytase gene having a nucleotide sequence as shown in SEQ ID NO. 7 and a signal peptide having a nucleotide sequence as shown in SEQ ID NO. 12.

Expression of phytase in

Provided is an &#x3b1; amylase variant obtained by the mutation or deletion of at least one amino acid residue in the amino acid sequence of a parent &#x3b1; amylase, and at the same time, same is an &#x3b1; amylase maintaining the capability of the parent to hydrolyse &#x3b1;-1,4 glycosidic bond, wherein the amino acid sequence homology of the two reaches 95% or more. A series of &#x3b1; amylase variants provided therein have a relatively high catalytic activity under acidic conditions of pH 5.0 and high temperatures of at least 100&#xb0; C.

&#x3b1;-amylase variant and use thereof

The present invention discloses an L-type amylase variant and use thereof. The &#x3b1;-amylase variant is obtained by deleting the first N-terminal amino acid residue V from the &#x3b1;-amylase ofand replacing it with three other amino acid residues DGL. The &#x3b1;-amylase variant provided by the present invention has high catalytic activity under the acidic conditions of pH 5.0-5.8 and a high temperature of 100&#xb0; C. or above. The acid resistance and thermal stability of these &#x3b1;-amylase variants are suitable for starch liquefaction.

L-type amylase variant and use thereof

Growing community of inventors

Nanjing, China

Yan Sun