Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Yan Li

Wei-min Liu

Yu Chuan Tai

Rajiv Dua

Christopher David Santini

Marcel Fontecha

Lori Steiner

Xiaoying Chen

Xiaoju (Max) Ma

Sim-Jasmine Truong

Chih-Jian Lih

Bryan Hoglund

Janet Jin

Christopher Santini

Provided herein are methods and compositions to classify DLBCL subtypes using quantitative RT-PCR.

Methods and compositions for classifying DLBCL

Accurate and fast mapping of sequencing reads obtained from a targeted sequencing procedure can be provided. Once a target region is selected, alternate regions of the genome that are sufficiently similar to the target region can be identified. If a sequencing read is more similar to the target region than to an alternate region, then the read can be determined as aligning to the target region. The reads aligning to the target region can then be analyzed to determine whether a mutation exists in the target region. Accordingly, a sequencing read can be compared to the target region and the corresponding alternate regions, and not to the entire genome, thereby providing computational efficiency.

Accurate and fast mapping of reads to genome

A new in-frame deletion was found in exon 19 of the EGFR gene, the exon that is often mutated in tumors. The invention comprises methods of detecting the mutation, methods of prognosis and methods of predicting response to treatment based on the presence of absence of the mutation.

Mutation in the epidermal growth factor receptor kinase domain

This invention provides methods, compositions, and kits relating to biomarkers whose expression levels are correlated with diffuse large B-cell lymphoma (DLCBL) patients' response to treatment with a CD20 antagonist, such as a CD20 antibody, exemplified by rituximab. The methods, compositions, and kits of the invention can be used to identify DLBCL patients who are likely or not likely, to respond to anti-CD20 treatments.

Predicting response to anti-CD20 therapy in DLBCL patients

Growing community of inventors

Palo Alto, CA, United States of America

Yan Li