Temasek Life Sciences Laboratory Limited

Joil (s) Pte Ltd.

Yan Hong

Srinivasan Ramachandran

Deyu Xie

Xiao Liu

Peter Leong Bin Chia

The present invention is directed to a self-quenching primer comprising; a fluorophore that can be quenched by guanine; an oligonucleotide sequence that forms a hairpin; an oligonucleotide that is a target specific sequence and; use in amplification reactions, particularly in polymerase chain reactions, during which the fluorophore is released thereby emitting fluorescence.

Fluorescent primer system for detection of nucleic acids (Q priming)

The present invention is directed to a method ofregeneration through organogenesis and a method of genetic transformation of. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced toviainfection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.

[object Object]

Regeneration and genetic transformation of Acacia mangium

Growing community of inventors

Singapore, Singapore

Yan Hong