Cp Kelco U.s., Inc.

Fundacion Instituto Leloir

Yamini N Patel

Nancy E Harding

Russell J Coleman

Neil Argo Morrison

Ellen Burke

J Carrie Schneider

Ralph Bezanson

Dagmar Meissner

Stan Bower

Steven Matzke

Jane C Schneider

Maria Veronica Ielmini

Luis Ielpi

Todd A Talashek

Joseph M Cleary

Todd Talashek

Russel Coleman

Luis Lelpi

Maria Veronica Lelmini

Jeff Tutak

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene N, M or Istrains containing at least one genetic modification that favors slime-forming polysaccharide production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

Targeted gene deletions for polysaccharide slime formers

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative ofsp. ATCC 53159 that harbors a multicopy broad host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineeredstrain including the needed DNA sequence is encompassed within this invention.

Modified organisms for producing gums

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R, M or Nstrains containing at least one genetic modification that favors slime-forming polysaccharide production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The invention relates to mutant strains of the genuswhich have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate ('PHB') synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PHB-deficient Sphingan solution, to a clarification temperature of about 30&#xb0; C. to about 70&#xb0; C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

Mutant bacterial strains of the genus sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof

PHB-deficientstrains having improved sphingan yield are provided. Certain of thestrains are diutan-producing strains that exhibit a dramatic improvement in productivity and yield due to a combination of certain genetic modifications that affect PHB and sphingan synthesis. Moreover, the sphingans produced from such strains have superior characteristics including improved filterability, clarity, and improved rheology-modifying characteristics. The sphingans provided are, thus, highly desirable in a variety of commercial and industrial uses, including personal care items, cement applications, and oilfield applications.

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The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R, M or Nstrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative ofsp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineeredstrain including the needed DNA sequence is encompassed within this invention.

Method of producing a diutan gum

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene Istrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene Rstrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene M or gene Nstrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The invention relates to mutant strains of the genuswhich have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate ('PHB') synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PUB-deficient Sphingan solution, to a clarification temperature of about 30&#xb0; C. to about 70&#xb0; C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative ofsp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the hoststrain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineeredstrain including the needed DNA sequence is encompassed within this invention.

Growing community of inventors

San Diego, CA, United States of America

Yamini N Patel