Allosource

Lonza Walkersville, Inc.

Octane Biotech Inc.

Lonza Cologne Gmbh

Yaling Shi

Eytan Abraham

Carolyn Barrett

Timothy Smith

Nuala Trainor

Samatha Bandapalle

Joseph O'Connor

Kelly Purpura

Erika McAfee

Timo Gleissner

Ann Siehoff

Brent Lee Atkinson

Reginald L Stilwell

Carolyn Barrett Rorick

Brian Dittman

Joseph O'connor

Matthew Hewitt

Chase McRobie

Brent Atkinson

The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.

Cell culture chamber with improved cell-contacting surfaces

Compositions comprising a cartilage sheet comprising a plurality of interconnected cartilage tiles and a biocompatible carrier are provided. Methods of manufacturing cartilage compositions comprising a cartilage sheet comprising a plurality of interconnected cartilage tiles are also provided.

Cartilage mosaic compositions and methods

The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.

End-to-end cell therapy automation

The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.

Cell concentration methods and devices for use in automated bioreactors

The present invention provides compositions for treating soft tissue injuries comprising a collagen matrix and mesenchymal stem cells adhered to the collagen matrix. Methods of making and using compositions comprising a collagen matrix and mesenchymal stem cells adhered to the collagen matrix are also provided.

Cell repopulated collagen matrix for soft tissue repair and regeneration

There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose tissue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the substrate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove unwanted cells from the substrate. Other embodiments are also disclosed.

Allografts combined with tissue derived stem cells for bone healing

Osteochondral graft composition that include a cartilage component and a bone component, and which include one or more perforations in the bone component and/or the cartilage component, are provided. Methods of manufacturing and using such osteochondral graft compositions are also provided.

Methods of manufacturing perforated osteochondral allograft compositions

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Larkspur, CO, United States of America

Yaling Shi