Life Technologies Corporation

Cygnus Biosciences (Beijing) Co., Ltd.

Cygnus Biosciences Co. Ltd.

Yalei Wu

Chunmei Liu

Kai Qin Lao

Caifu Chen

Shoulian Dong

Linda Wong

Wai Ho Lee

David Keys

Genhua Zheng

Provided herein are methods, compositions, and kits for forming amplification products. In various embodiments provided herein, transposomes comprising transposases are used in forming tagged polynucleotides for downstream amplification and polynucleotide processing steps.

Methods and compositions for preparing polynucleotides

Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5' and 3&#x2032; ligation adaptors to the 5&#x2032; and 3&#x2032; ends of the mature small RNAs, respectively, in the presence of 5&#x2032; and 3&#x2032; semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3&#x2032; end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

Small RNA capture, detection and quantification

A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

Methods and systems for PCR quantitation

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Foster City, CA, United States of America

Yalei Wu