Schering Corporation

Schering-plough Corporation

Yair Alroy

Paul J Leibowitz

Russell Gg Condon

Allan Morenberg

Steven Blaisdell

Schaefer Eugene

Jingdong Zhu

Process for recovering lipophilic oligosaccharide antibiotics

An insoluble mammalian protein is extracted from transformed bacteria expressing the mammalian protein while avoiding irreversible insolubilization of bacterial host proteins by homogenizing the fermentation broth, centrifuging the homogenized broth and removing the supernatant liquid for the inclusion body containing pellet. In another embodiment, the pH of the homogenized broth is adjusted to 2.0 prior to centrifugation. The acidified broth is then centrifuged, and the pellet is resuspended in buffer, homogenized again, and the inclusion body is isolated by centrifugation.

Extraction of heterologous insoluble proteins from bacteria

Extraction of granulocyte macrophage colony stimulating factor from

A method is provided for extracting human interleukin-4 (IL-4) from IL-4 expressing bacterial cells. The method comprises treating the bacterial cells with a deactivating agent, disrupting the cell membrane and recovering the IL-4.

Extraction of human interleukin-4- from bacteria

A method of extracting granulocyte macrophage colony stimulating factor (GM-CSF) from GM-CSF-expressing bacterial cells comprising treating a suspension of GM-CSF-containing bacterial cells with an acid and an enhancing agent, removing substantially all of the suspension liquid from the cells, preparing a second suspension of the acidified cells, neutralizing said second suspension, and separating the GM-CSF containing liquid from the suspended cells.

Growing community of inventors

Parsippany, NJ, United States of America

Yair Alroy