Bio-rad Laboratories, Inc.

Anchor Molecular Inc.

Yabin Lu

Roger P Walker

Urvee Desai

Daming Shan

Robert K Likuski

Michael Jinyang Lu

Roger Walker

Disclosed herein is a method which includes extracting genomic deoxyribonucleic acid (DNA) at locations at or near cancer hotspots from a subject, modifying Tier-1 5hmC on the DNA to a modified 5hmC, detecting and identifying the presence or absence of the modified 5hmC, quantifying the detected and identified modified 5hmC; and providing a report comprising a score, wherein the score is indicative of the presence of cancer.

Method for detecting cancer using 5-hydroxymethylcytosine (5-hmC)

The invention is directed to methods and kits which utilize an immobilized solid support to extract the nucleic acids present in the human plasma sample Immobilized solid support further comprises: a) a pre-treated solid support material b) M-Aminophenylboronic acid (APBA), and c) levoglucosenone. The components and methods for activating solid support and extraction of nucleic acids used in the invention provide for high extraction efficiency and the resultant product is a highly purified plasma which is almost completely free of nucleic acids.

Method of removing nucleic acids from human plasma

Methods of diagnosing celiac disease in a subject are provided. In some embodiments, the method comprises contacting a sample of bodily fluid from the subject with an antigen comprising a recombinant or synthetic deamidated gliadin protein, wherein the deamidated gliadin protein comprises a hexamer of peptides each having the sequence of SEQ ID NO:1; and detecting an antibody from the sample that specifically binds to the antigen, thereby diagnosing celiac disease in the subject.

Recombinant deamidated gliadin antigen

The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Methods, systems, and apparatus for accurately determining a proportion (ratio) of two analytes is provided, as well as provide a concentration of a first analyte from a determined concentration of a second analyte and from a proportion of the analytes to each other. In one aspect, a surface model (called a 'dose surface' herein) relating the concentrations of the two analytes to the proportion can be used to obtain accurate values for one of the variables (e.g. a concentration or the proportion) when the other two variables have previously been obtained. The dose surface can be a three-dimensional surface and be non-linear. The dose surface model can include multiple regression functions. For example, measured responses can be individually converted to concentrations using two dose-response curves, and the concentrations can be input to a dose surface function to obtain the proportion.

Dose surface method for determination of analyte ratios

The ratio of analytes is determined directly from the responses of the analytes using a conversion method. Individual analyte responses are obtained by using a selected measuring technique, and these individual responses are used as the independent variables in a conversion method. The dependent variable of conversion method is the desired analyte ratio. The resulting conversion method is then used to directly calculate the desired ratio of analytes as a function of the measured responses. No intermediate conversions, such as using a calibration curve to convert individual measured analyte responses to concentration values, are needed to obtain the desired ratio.

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Pleasanton, CA, United States of America

Yabin Lu