Dongguan Apac Biotechnology Co., Ltd.

Genozyme Biotech, Inc.

Ya-Shan Cheng

Cheng-Yen Lin

Hui-Lin Lai

Tzu-Hui Wu

Rey-Ting Guo

Jian-Wen Huang

Ting-Yung Huang

Tsung-Yu Ko

Chun-Chi Chen

I-Hsuan Lin

Cheng-Bin Zheng

A beta-glucosidase having improved enzymatic activity is disclosed. The amino acid sequence of the beta-glucosidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of tyrosine at position 286 with phenylalanine, or a substitution of asparagine at position 639 with glutamate.

Beta-glucosidase having improved enzymatic activity

A xylosidase having improved enzymatic activity is disclosed. The amino acid sequence of the xylosidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of phenylalanine at position 35 with glutamate, and/or a substitution of glutamine at position 41 with histidine.

Xylosidase having improved enzymatic activity

A glucose oxidase having improved thermostability is disclosed. The amino acid sequence of the glucose oxidase is a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of glutamate at position 129 with proline, and/or a substitution of glutamine at position 243 with valine.

Glucose oxidase having improved thermostability

A phytase having improved thermostability is disclosed. The phytase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is one of mutations A to D. The mutation A is to substitute amino acids at positions 143 and 262 with cysteine, the mutation B is to substitute amino acids at positions 259 and 312 with cysteine, the mutation C is to substitute amino acids at positions 205 and 257 with cysteine, and the mutation D is to substitute amino acids at positions 264 and 309 with cysteine.

Phytase having improved thermostability

A cellulase having improved thermostability is disclosed. The cellulase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is adding a cysteine in N terminal and adding a glycine and a cysteine or adding a proline and a cysteine in C terminal.

Cellulase having improved thermostability

A cellulase having improved enzymatic activity is disclosed. The cellulase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 161 with Histidine.

Cellulase having improved enzymatic activity

A pectinase having improved thermostability is disclosed. The pectinase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of serine at position 181 with phenylalanine, methionine or leucine.

Thermostable pectinase

A phytase having improved enzymatic activity is disclosed. The phytase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Valine at position 90 with Threonine. Alternatively, the phytase has a modified amino acid sequence of SEQ ID NO: 6, wherein the modification is a substitution of Asparagine at position 204 with Alanine or a substitution of Serine at position 206 with Alanine.

Phytase having improved enzymatic activity

A &#x3b2;-mannanase having increased production yield and enzymatic activity is disclosed. The &#x3b2;-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 25 with Histidine.

&#x3b2;-mannanase having improved production yields and enzymatic activity

A &#x3b2;-mannanase having increased enzymaic activity is disclosed. The &#x3b2;-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.

&#x3b2;-mannanase having improved enzymatic activity

A xylanase having increased enzymaic activity is disclosed. The xylanase has a modified amino acid sequence of SEQ ID NO: 2, wherein Tryptophan at position 125 is substituted with Phenylalanine and Phenylalanine at position 163 is substituted with Tryptophan.

Xylanase having improved enzymatic activity

A glucanase having increased enzyme activity and thermostability is disclosed. The glucanase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein valine at position 18 is substituted with tyrosine and tryptophan at position 203 is substituted with tyrosine.

Glucanase having increased enzyme activity and thermostability

A thermostable cellulase having increased enzyme activity is disclosed. The cellulase comprises a modified amino acid sequence of SEQ ID NO: 2, wherein the tyrosine at position 61 is substituted with an amino acid selected from the group consisting of phenylalanine, alanine and glycine.

Growing community of inventors

Taipei, Taiwan

Ya-Shan Cheng