Mgi Tech Co., Ltd.

Bgi Shenzhen

Ya Gao

Hui Fu Jiang

Fang Zhou Chen

Lin Yang

Yanyan Zhang

Xun Xu

Peng Zeng

Yuqian Wang

Guodong Huang

Jing Guo

Fang Chen

Haojun Jiang

Haiping Zhang

Jia Zhao

Yicong Wang

Sen Lu

Na Zhong

Xuehan Zhuang

Hui Jiang

Shaohong Zhang

Yifan Xie

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3' end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

PCR primer pair and application thereof

Provided is a PCR primer pair, comprising: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on 3' end of the first primer, and the first random sequence is located on 5&#x2032; end of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on 3&#x2032; end of the second primer, and the second random sequence is located on 5&#x2032; end of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence respectively, and the first random sequence and the second random sequence are inverse complementary.

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3' of the first primer, and the first random sequence is located on end 5&#x2032; of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3&#x2032; of the second primer, and the second random sequence is located on end 5&#x2032; of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.

Provided is a method for isolating exosomal DNA, the method comprising: (i) providing a sample, wherein the sample comprises a blood sample from peripheral blood of a pregnant woman; and (ii) subjecting the sample to isolating, thus obtaining the exosomal DNA. Also provided are a method for detecting a blood sample, a method for constructing a sequencing library on a blood sample, a method for high-throughput sequencing an exosomal DNA sequencing library, a method for non-invasive prenatal gene detection, a device for non-invasive prenatal gene detection, and a kit for detecting a blood sample and use thereof.

Method for non-invasive prenatal diagnosis based on exosomal DNA and application thereof

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Shenzhen, China

Ya Gao