Basf Se Corporation

Basf Enzymes LLC

Verenium Corporation

Dsm IP Assets B.v.

Xuqiu Tan

Adrienne Huston Davenport

Arne I Solbak, Jr

Jochen Kutscher

Jared Dennis

Mark Joseph Burk

Dan Eric Robertson

Nelson Robert Barton

Stefan Haefner

David E Lam

Robert Christopher Brown

Geoff Hazlewood

Joel Kreps

Giselle G Janssen

Kenneth Barrett

Amit Vasavada

Svetlana Gramatikova

Andreas Funke

Charles Isaac

Wilhelmus P Van Hoek

Adrian Badillo

Bin Zhang

Richard S Lee

Katie Kline

Claudia Esper

Stefan Jenewein

Cristina Pop

Kristine Chang

Oliver Spangenberg

Stefan Seemayer

Michael Seitter

Hugo D Urbina

Michael Liszka

Tong Li

Giselle Janssen

Roderick James Fielding

Jincai Li

Blake G Sturgis

Asfia Qureshi

Melissa Ann Scranton

Robin Cai

Anh-Huy Phan Le

Hugo Urbina

Corey Dodge

Jingping Zhong

Michael Speer

Ruorong Cai

Amy Migliori

Dongmei Ren

Roderick Fielding

Kenneth E Barret

Robert C Brown

Michael Friedrich Hermann Seitter

Samantha Orchard

Tuan Tran

Gina Ning Lee Lowe

Arne I Solbak

Michael Friedrich Seitter

Joel A Kreps

Aileen Aboy Milan

Kevin Li Wong

Kristine W Chang

The present invention relates to variants of an alpha-amylase which have an increased exoamylase activity compared to the parent alpha-amylase. The present invention also relates to methods of making the variant alpha-amylase and the use of the variant alpha-amylase in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol or syrups and as viscosity breaker in oilfield and mining industries.

Amylase enzymes

Disclosed herein is an improved Gene Site Saturation Mutagenesis (GSSM) method for producing a plurality of modified polynucleotides and/or polypeptides, creating specific changes to a gene, and reassembling mutations or changes at one or more sites.

Gene site saturation mutagenesis (GSSM) method

A promoter operably linked to a gene encoding a protein is disclosed. The promoter drives expression of the protein in a yeast cell in the absence of methanol. Also disclosed are vectors, host cells and expression systems that include the promoter, as well as methods of using the promoter to express proteins in yeast.

Promoter for yeast

Engineered variant polypeptides having lipase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered lipase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, biodiesel and ethanol production.

Lipase enzymes

Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis ('GSSM') method or the tailored multi-site combinatorial assembly ('TMCA') method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.

Nucleases and methods for making and using them

Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

Beta-amylase enzymes

Lipase enzymes and methods of using the lipases in a baking for improving the volume, stability, tolerance of a baked product and/or reducing and reducing or eliminating the use of DATEM.

Baking lipase and methods of use

Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/L to 40 g/L. The phytase have higher specific activity, retain activity at low pH, and retain activity at high temperature. The phytase can be used in a variety of compositions including food, feed, pharmaceuticals, and cleaning.

Phytase

The present disclosure provides for a polynucleotide sequences encoding a xylanase. More specifically, the present disclosure provides for polynucleotide sequences with codon mutations encoding a xylanase.

Genes with codon mutations encoding xylanase

Polynucleotide sequences are provided encoding a thermostable cellulase and directing its increased expression are provided, and the use of the thermostable cellulase in hydraulic fracturing methods and the treatment of flowback fluids.

Gene encoding cellulase

The present invention provides a novel method of producing a plurality of modified polynucleotides having different combinations of various mutations at multiple sites by a tailored multi-site combinatorial assembly, comprising adding at least two or at least three primers to a double stranded template polynucleotide in a single reaction mixture, wherein the primers are not overlapping, and wherein each of the primers comprise at least one mutation different from the other primers, wherein at least one primer is a forward primer that can anneal to a minus strand of the template and at least one primer is a reverse primer that can anneal to a plus strand of the template, and subjecting the reaction mixture to a polymerase extension reaction to yield a plurality of extended modified polynucleotides from the at least three primers. The method can be performed without employing a ligation step prior to transforming the extended modified polynucleotides into a cell. The plurality of extended modified polynucleotides can be treated with an enzyme for destroying the template polynucleotide prior to transforming in to the cell.

Tailored multi-site combinatorial assembly

The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.

Phospholipases, nucleic acids encoding them and methods for making and using them

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San Diego, CA, United States of America

Xuqiu Tan