Mgi Tech Co., Ltd.

Bgi Shenzhen

Shenzhen Leben Technology Co., Ltd.

Complete Genomics, Inc.

Merck Sharp + Dohme Corp.

Fudan University

Bgi-Shenzhen Co., Limited

Egi Tech (Shen Zhen) Co., Limited

Beijing Genomics Institute at Shenzhen

Bgi Genomics Co., Limited

Xun Xu

Jun Wang

Ming Ni

Wenwei Zhang

Zhiping Liu

Radoje Drmanac

Snezana Drmanac

Handong Li

Wei Ma

Dong Wei

Ao Chen

Jiabo Wu

Jiansheng Tang

Lin Yang

Jin Yang

Hui Xing Wang

Qian Wang

Xin Liu

Jian Wang

Hui Fu Jiang

Matthew J Callow

Fang Zhou Chen

Chongjun Xu

Yanyan Zhang

Naibo Yang

Ya Gao

Yong Liu

Ye Tao

Mei Li

Yue Shen

Yun Wang

Wei He

Shuangshuang Li

Jing Guo

Lili Wang

Fan Zhou

Liang Wu

Bin Xie

Yong Hou

Wenbin Chen

Xiaoyu Liu

Xiuqing Zhang

Huanming Yang

Peng Zeng

Himanshu Sethi

Peter M Shaw

Yuqian Wang

Shihong Chen

Erkai Liu

Fang Chen

Leon Eckhardt

Weimao Wang

Guodong Huang

Haojun Jiang

Xuyang Yin

Mengzhe Shen

Xin Fang

Devan V Mehrotra

Hui Wang

Lingling Peng

Junhua Li

Scott Gablenz

Xiaoqing Liu

Jingyi Cheng

Lijia Tian

Ruiqi Xu

Zhuokun Li

Shengming Zhao

Dennis G Ballinger

Weiming He

Kang Kang

Yicong Wang

Na Zhong

Chuanyu Liu

Yue Yuan

Longqi Liu

Hanjie Wu

Zhouchun Shang

Xuehan Zhuang

Rebecca L Blanchard

Robin Mogg

Mary Beth Dorr

Yuxiang Li

Li Bao

Jielei Ni

Yuanqing Liang

Judong Shen

Zeyu Su

Ke Ji

Mengnan Cheng

Liqin Xu

Yuxuan Liu

Youwen Guo

Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S).

Magnetic bead-based detection method, storage medium, and detection device

Disclosed are a super-resolution imaging system (), a super-resolution imaging method, a biological sample identification system () and method, a nucleic acid sequencing imaging system () and method, and a nucleic acid identification system () and method. The super-resolution imaging system () includes an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) includes an excitation light source () and a structured light generation and modulation device (). The excitation light source () outputs the excitation light, and the structured light generation and modulation device () modulates the excitation light into structured light to irradiate the biological sample to generate the excited light.

Super-resolution imaging system and method, and nucleic acid sequencing imaging system and method

A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

Gene sequencing reaction device, gene sequencing system, and gene sequencing reaction method

A nucleic acid synthesis device and a nucleic acid purification device, uses thereof, and a nucleic acid synthesis method and a nucleic acid purification method. The nucleic acid synthesis device includes a solid support, and the solid support includes a controlled pore glass (CPG), the CPG is an unmodified and bare CPG, a surface of the CPG has a hydroxyl group, and the hydroxyl group is attachable, though a covalent bond, to a phosphoramidite-protected nucleotide monomer or multimer for synthesis of nucleic acid. The nucleic acid synthesis device of the present disclosure can be used for not only synthesis of an oligonucleotide primer, but also for purification of enzymatic digestion and PCR product by using the oligonucleotide primer immobilized on the CPG, and has advantages of simple structure, small volume, light weight, high efficiency, low costs, and diversified functions.

Nucleic acid synthesis and purification device, use thereof, and nucleic acid synthesis and purification method

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3' end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

PCR primer pair and application thereof

The present invention provides genetic markers on human chromosome 6 that are associated with a beneficial response to a treatment that targets() toxin B (TcdB), e.g. a TcdB antibody. These TcdB treatment response markers are useful, inter alia, to identify patients who are most likely to benefit from treatment that targets TcdB in methods of treating patients having a disease susceptible to treatment with a TcdB antibody, and in methods for selecting the most appropriate therapy for such patients. The invention also provides antibodies, drug products, and kits useful with the TcdB Treatment response markers of the invention.

Human genetic markers associated with response to treatments that target clostridium difficile toxin B

The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

Nucleic acid sequencing using affinity reagents

Provided is a PCR primer pair, comprising: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on 3' end of the first primer, and the first random sequence is located on 5&#x2032; end of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on 3&#x2032; end of the second primer, and the second random sequence is located on 5&#x2032; end of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence respectively, and the first random sequence and the second random sequence are inverse complementary.

The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

Nucleic acid sequencing method

The present disclosure proposes a modeling method and apparatus for diagnosing an ophthalmic disease based on artificial intelligence, and a storage medium. The modeling method includes: establishing a data collection of ophthalmic images and a data collection of non-image ophthalmic disease diagnosis questionnaires; training a first neural network model by employing the data collection of the ophthalmic images to obtain a first classification model; training a second classification model by employing the data collection of non-image ophthalmic disease diagnosis questionnaires; and merging the first classification model and the second classification model to obtain a target classification network model, in which, a test result outputted by the target classification network model is used as a diagnosis result of the ophthalmic disease.

Modeling method and apparatus for diagnosing ophthalmic disease based on artificial intelligence, and storage medium

The present invention provides a method for improving the loading of nucleic acid on a solid support by contacting the solid support with a poloxamer-containing reagent. The present invention also provides a method for improving the stability of a nucleic acid on a solid support, comprising contacting a nucleic acid molecule with a partially double-strand oligonucleotide before or after loading the nucleic acid molecule on a solid support, so as to cause the nucleic acid molecule to hybridize with the oligonucleotide. The present invention also provides a combined use of the two methods.

Method for improving loading and stability of nucleic acid

Provided is a single fluorescent dye based sequencing method. Moreover, the present invention further provide modified nucleosides and nucleotides, and a kit comprising the nucleoside and/or nucleotide, particularly suitable for the sequencing method of the present invention. Additionally, the present invention further provides uses of the nucleoside, the nucleotide and the kit for sequencing.

Single fluorescent dye-based sequencing method

Provided in the present invention is a method for constructing single cell sequencing libraries, comprising the following steps: a) lysing a single cell to obtain a single cell lysate; b) separating the nucleus and the cytoplasm in the single cell lysate obtained in step a) to obtain a nuclear solution and a total RNA solution; and c) constructing a chromatin DNA library with the nuclear solution obtained in step b) to obtain a chromatin-accessibility sequencing library of the single cell; and constructing a transcriptome library with the total RNA solution obtained in step b) to obtain a transcriptome sequencing library of the single cell.

Method for constructing single cell sequencing library and use thereof

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Shenzhen, China

Xun Xu