Anhui Agricultural University

Yingshang Shili Ecological Agriculture Technology Co., Ltd.

Xueqing Liu

Ying Wang

Xiaoyan Tang

Chenggang Wang

Ting Li

Guohu Chen

Qian Yin

Hongwei Wen

Siwen Wu

A method for obtaining regenerated seedlings ofL. ssp.from embryonic tip tissues, including the following steps. A seed is inoculated to a germination medium for dark culture for 60 h. The testa, root tip, two cotyledons and middle growing point of the resultant germinated seed are removed, and an embryonic tip with a length of 3-5 mm is retained as an explant. The explant is sequentially subjected to low-temperature pre-culture in a pre-culture medium for 36 h, room-temperature shaking culture in a liquid bud induction culture medium for 10 min, and bud induction culture in a bud induction culture medium for 20 d. A regenerated plant with 5-6 leaves is transferred to a rooting medium for rooting culture for about 2 weeks, and a well-rooted plant is collected, and subjected to hardening and transplantation into a filed.

Method for obtaining regenerated seedlings offrom embryonic tip tissue

Growing community of inventors

Hefei, China

Xueqing Liu