Bio-rad Laboratories, Inc.

Xuemei Yang

Cory Panattoni

Lee Olech

Shane Petersen

Craig Rowell

Sean Cater

Thomas R Berkelman

Christopher Belisle

Lei Li

Tom R Berkelman

Cory M Panattoni

Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Hydrolysis-resistant polyacrylamide gels

Hand cast gels, and solutions for their preparation, are described.

Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability

Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.

Polymeric sorbent sheets as ion reservoirs for electroblotting

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Walnut Creek, CA, United States of America

Xuemei Yang