Chinese Academy of Sciences

University of Florida Research Foundation Inc

Chinese Academy of Medical Sciences

Xueli Zhang

Keelnatham T Shanmugam

Lonnie O'Neal Ingram

Xinna Zhu

Hongtao Xu

Yanhe Ma

Dong Wang

Jonathan C Moore

Luqi Huang

Lili Zhang

Juan Guo

Xuan Wang

Zhubo Dai

Yi Liu

Kaemwich Jantama

Jinlei Tang

Tao Sun

Lorraine P Yomano

Jing Zhao

Jing Ran Chen

Elliot N Miller

Laura R Jarboe

Guanping Dai

Mark John Haupt

Zaigao Tan

Pingping Liu

Qingyan Li

Dr Lonnie O'Neal Ingram

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.

Recombinant yeast and use thereof

Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of &#x3b2;-elemene and/or germacrene A.

This invention relates to biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulation and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yields in mineral salts media during simple pH-controlled batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenolpyruvate carboxykinase (pck) derepressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such asand, with one difference: that the biocatalysts are able to achieve this high level of succinic acid production in a minimal salt medium with carbohydrate source, as opposed to the requirement for a rich medium for succinic acid production by rumen bacteria.

Engineering the pathway for succinate production

The invention relates to the field of producing succinate byfermentation. Specifically, the invention provides an engineered recombinantfor producing succinate, wherein saidcontains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinantfor producing succinate, and a method of using the engineered recombinantfor producing succinate.

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Provided is recombinantcomprising a mutated ldhA gene. Disclosed are use of the recombinantin the preparation of D-lactate, and method of preparing D-lactate by employing the recombinant. In the method of preparing D-lactate, fermentation can be conducted at a high temperature (42-50&#xb0; C.), and aqueous ammonia or sodium hydroxide can be used as a neutralizer during the fermentation.

Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase inor the mutant thereof. The method for improving the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase inor the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) inor the mutant thereof with any of the following regulating elements: artificial regulating element M1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of &#x3b1;-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved.

Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism

The invention relates to the field of modifyingthrough genetic engineering. Specifically, the invention provides ancontaining a mutated lpdA gene. The invention also relates to use of thein the production of chemical material such as ethanol, and succinate etc. The invention also provides a method of producing chemical materials such as ethanol and succinate etc. by using the, as well as a method for increasing the activity of pyruvate dehydrogenase inby introducing a mutated lpdA gene.

A process for the production of malic acid in commercially significant quantities from the carbon compounds by genetically modified bacterial strains (GMBS; also referred to as biocatalysts or genetically modified microorganisms) is disclosed. Microorganisms suitable for the production of malic acid can be cultured in one or two-step processes as disclosed herein.

The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.

Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms.

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Tianjin, China

Xueli Zhang