Syngenta Participations Ag

Xuejun Sean Zhong

Shib Sankar Basu

Yan Zhang

Aron Silverstone

Myoung Kim

Shengsheng Zhang

Rogerio T N Prata

Tricia Charlene Costello

Andrew DeBrecht

Hangsik Moon

Methods are disclosed for detecting and measuring polysaccharide-hydrolyzing enzyme activity or concentration by partial hydrolysis using a pre-determined, yet short, incubation time and a pre-determined temperature. The resulting reaction mixture has unique chemical (i.e., reaction products) and physical (i.e., viscosity) properties that can be used to detect or measure the polysaccharide-hydrolyzing enzyme activity or concentration.

Method of measuring alpha amylase

The present invention is directed to C-terminal truncated &#x3b1;-amylase polypeptides, nucleic acid sequences encoding the truncated &#x3b1;-amylase polypeptides, and methods of using the same. Further provided are expression cassettes, plants and plant parts expressing the nucleic acid sequence encoding the truncated &#x3b1;-amylase. Expression of the truncated &#x3b1;-amylase in a plant cell results in enhanced accumulation of biologically-active &#x3b1;-amylase, or enhanced secretion of the &#x3b1;-amylase from the plant cell, compared to the accumulation or secretion of a full length &#x3b1;-amylase.

Truncation of the C-terminal end of alpha-amylase

Growing community of inventors

Cary, NC, United States of America

Xuejun Sean Zhong