Other

Wuhan Grand Hoyo Co., Ltd.

Xuechen Mei

Jing Li

Kun Wan

Lei Huang

Panpan Xing

Haixia Su

Aifu Liu

Jiong Wang

Mengjun Song

Pei Fan

Jiaqi Huang

Lei Yang

Jiajun He

Liping Guo

Min Shu

Peng Tang

Chengjun Zhu

Provided is a method for screening aconstitutive expression vector promoter on the basis of transcriptome sequencing; and further provided are theconstitutive expression vector promoter screened on the basis of transcriptome sequencing, an expression vector comprising the promoter, a recombination strain obtained by transforming a host cellusing the expression vector, and applications thereof.

constitutive expression vector promoter screened on the basis of transcriptome sequencing, screening method thereof, and applications thereof

Disclosed is a L-isoleucine-producingfermentation medium, comprising a basal medium and a growth factor, wherein the growth factor consists of choline, betaine and vitamin B6, and the contents of each ingredient in the fermentation medium are: 0.2-1 g/L choline, 0.25-0.5 mg/L betaine, and 0.05-0.3 mg/L vitamin B6. Also disclosed is a method for cultivating the L-isoleucine-producing, comprising: inoculating the L-isoleucine-producingonto the fermentation medium, wherein the volume of the bacteria liquid accounts for 5-20% of the volume of the fermentation medium, adjusting the pH to 6.5-7 with aqueous ammonia, controlling the dissolved oxygen to 30-50%, and fermenting for 25-30 h; then decreasing the dissolved oxygen to 15-25%, and feeding a 50-80% glucose solution into the fermentation broth to control the residual sugar at 3-4%, continuing the fermentation until 60-70 hours, then terminating the fermentation, and controlling the temperature of the overall fermentation process at 29-33&#xb0; C.

L-isoleucine-producing corynebacterium glutamicum fermentation medium and culture method

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Wuhan, China

Xuechen Mei