Monsanto Technology LLC

Xudong Ye

Larry A Gilbertson

Michael W Petersen

Brian J Martinell

Yuechun Wan

Edward James Williams

Shihshieh Huang

David A Somers

Anatoly Rivlin

David A Walters

Paul S Chomet

Elysia K Krieger

Susan Jane Johnson

Erik D Dersch

Richard J Heinzen

Qi Wang

Ervin D Nagy

Kenneth A Barton

Yong Huang

Kenneth John Piller

Toni Armstrong

Junjiang Shen

Laura J Crow

Stanislaw Flasinski

Krishnakumar Sridharan

Yurong Chen

Wanggen Zhang

Jianping Xu

Ashok Shrawat

Susan Johnson

John D Bradley

David N Walters

David R Duncan

Richard J Lawrence

David Walters

Zarir E Vaghchhipawala

Sarah Heifner

Rebecca Rode

Lorena Moeller

Christine Shyu

Peizhen Yang

Annie Saltarikos

David Kelm

David Alan Somers

Elysia Krieger

Edward Williams

Anna Mary O'KEEFE

Shinhshieh Huang

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

Genome editing in plants

This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

Compositions and methods for genome editing in planta

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

Methods for plant transformation using spectinomycin selection

The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells.

Methods to improve site-directed integration frequency

The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

Methods for transforming corn explants

The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.

Methods and compositions for use in genome modification in plants

The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

Methods for transforming wheat explants and compositions therefor

The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

DNA constructs for obtaining marker-free transgenic plants

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

Method of meristem excision and transformation

The present invention relates to methods and compositions for improving the efficiency of- and-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

Use of multiple transformation enhancer sequences to improve plant transformation efficiency

Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

Vectors and methods for improved plant transformation efficiency

Growing community of inventors

Madison, WI, United States of America

Xudong Ye