Jiangsu University

Xucheng Zhou

Xiaobo Zou

Jiyong Shi

Xiaowei Huang

Zhihua Li

Xuetao Hu

Disclosed is a bacterial counting method, comprising the following steps, S1: preparing a polyaniline/bacterial composite film on the surface of a glassy carbon electrode by electric polymerization; S2: drawing the standard curve of the polyaniline/bacterial composite film modified electrode; and S3: determining the bacterial concentration of the bacteria solution sample to be tested according to the standard curve obtained in step S2. The method does not require the cultivation of the bacteria in the implementation process, such that same takes a short time and is easy to operate; the method uses aniline as the main reagent, and the consumption of the solution to be tested is small, such that the testing cost is low and the equipment is simple; the method has the advantages of a good repeatability and a wide detection linear range; and the method is an easy to operate, short time-consuming, and low cost bacterial counting method. The method is a bacterial counting method based on the polyaniline/bacterial composite film, and can achieve rapid and accurate detection of the bacterial thallus concentration.

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Jiangsu, China

Xucheng Zhou