Proteint (tianjin) Biotechnology Co., Ltd

Tianjin Nanuo Bio-technology Co., Ltd.

Xubo Yuan

Yunfeng Lu

Jie Li

Li Ren

Congcong Ma

Jin Zhao

Liangyu Chen

Chunsheng Kang

Lei Song

The present disclosure provides a method for increasing a number of proteins identified, including: S1. adding a binding buffer and an aluminosilicate zeolite to a sample to be tested to obtain a first suspension; S2. incubating the first suspension, subjecting an incubated first suspension to first high-speed centrifugation to obtain a first supernatant and a first precipitate, discarding the first supernatant, and retaining the first precipitate; S3. repeating a process as follows several times: resuspending the first precipitate with a washing buffer to obtain a second suspension, subjecting the second suspension to second high-speed centrifugation to obtain a second supernatant and a second precipitate, discarding the second supernatant, and retaining the second precipitate; and S4. preparing the second precipitate into a protein sample, and subjecting the protein sample to mass spectrometry detection.

Method for increasing number of proteins identified by mass spectrometry

Growing community of inventors

Tianjin, China

Xubo Yuan