Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Other

Virginia Tech Intellectual Properties, Inc.

Ei LLC

Corteva Agriscience LLC

Xu Hu

Guihua Lu

Dennis L Bidney

James Kevin Presnail

Feng Han

Oswald R Crasta

Michael Godwin

Soon-Chun Jeong

Alec A Hayes

Albert Laurence Lu

Xiaodan Yu

Scott H Diehn

Jonathan P Duvick

Joan Tellefsen Odell

Carol A Hendrick

M A Saghai Maroof

Nina Richtman

Lu Liu

Ute Schellenberger

Jon Duvick

Gusui Wu

Gary A Sandahl

Xiping Niu

Wolfgang Schuh

Julie Qi

Michelle Van Allen

Lisa Procyk

Zhangmeng Bao

Richard L Ruff

Jennifer S Tallman

Ghihua Lu

Ma Saghai Maroff

Yaxin Li

Jonathan William Robeson

Adane Kassa

Molly Mary McMahon

Bliss Marie Kernodle

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with one or more nucleic acid sequence encoding insecticidal protein(s) and one or more silencing element(s). In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The molecular and breeding stacks find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Insecticidal combinations of PIP-72 and methods of use

The present invention relates generally to methods of molecular biology and gene silencing to control pests.

Compositions and methods to control insect pests

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or aplant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiments, the pest is, or. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

A method for improving the sensitivity of an assay to determine the pathogenicity of a plant root pathogen using a soil amendment is presented. The method involves growing the plant root in the presence of a soil amendment after exposure of the plant root to the pathogen. A method of breeding plants is also provided.

Assay for plant root pathogen activity

The invention provides novel molecular genetic markers in soybean, where the markers are useful, for example, in the marker-assisted selection of gene alleles that impart disease-resistance, thereby allowing the identification and selection of a disease-resistant plant. The markers also find use in positional cloning of disease-resistance genes.

Marker mapping and resistance gene associations in soybean

This invention relates to an isolated isoflavone synthase 1 (IFS1) promoter nucleic acid fragment. The invention also relates to the construction of chimeric genes comprising all or a portion of the IFS1 promoter directing the expression of transgenes, in sense or antisense orientation.

Root-specific, stimulant inducible promoter and its use

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred and inducible promoter for the gene encoding a soybean ripening-related protein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Soybean root-preferred, nematode-inducible promoter and methods of use

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred and inducible promoter for the gene encoding a soybean dirigent protein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Root-preferred, nematode-inducible soybean promoter and its use

The present invention relates to nematode-regulated promoter and polypeptide-encoding nucleotide sequences and their use in creating or enhancing nematode-resistance in plants. Nucleic acid molecules comprising a nematode-resistance sequence operably linked to a nematode-regulated promoter and vectors, plant cells, plants, and transformed seeds containing such constructs are provided. Methods for the use of such constructs in repressing or inducing expression of a nematode-resistance sequence in a plant are also provided. In addition, methods are provided for conferring or improving nematode resistance in plants by repression or induction of nematode-resistance sequences.

Isolated nematode regulated gene promoter and use thereof

The present invention relates to nematode-regulated promoter sequences and their use in creating or enhancing nematode-resistance in plants. Nucleic acid molecules comprising a heterologous nucleotide sequence operably linked to a nematode-regulated promoter and vectors, plant cells, plants, and transformed seeds containing such constructs are provided. Methods for the creation and use of such promoters in repressing or inducing expression of a heterologous nucleotide sequence in a plant, as well as methods for creating or enhancing nematode-resistance in plants by such repression or induction of heterologous nucleotide sequences by nematode-regulated promoters are also provided.

Compositions and methods for promoting expression of nucleic acids in plants

Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a LOX protein are provided. Nucleotide sequences comprising the LOX promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.

Sunflower LOX polynucleotides and related compositions

The invention relates to isolated sunflower nucleic acid sequences encoding a protein having antipathogenic activity, vectors, plant cells, plants and seeds comprising said nucleic acid sequences. The invention further relates to a method of transforming plants for increased resistance against plant pathogens.

Sunflower anti-pathogene proteins and genes and their uses

Growing community of inventors

Johnston, IA, United States of America

Xu Hu