Sansure Biotech Inc.

Xu Fan

Lizhong Dai

Ling Wu

Guo Fu

Deyong Tan

Long Peng

Jia Liu

Zhongping Deng

Bozhi Ji

A rapid amplification method for a nucleic acid of hepatitis B virus, comprises the following steps: mixing a sample containing hepatitis B virus with a nucleic acid releasing agent, and adding a PCR premix to obtain a reaction solution, the PCR premix comprising deoxyribonucleoside triphosphate, an upstream primer as shown in the sequence SEQ ID NO: 1, a downstream primer as shown in the sequence SEQ ID NO: 2, a DNA polymerase and an amplification buffer; placing the reaction solution in a PCR reaction tube so that the reaction solution is in a form of a thin film with a thickness of 0.1 mm or less; performing PCR amplification under the following reaction condition: pre-denaturation at 90 to 100° C. for 10 s to 600 s, denaturation at 90 to 100° C. for 0 to 1 s, and annealing and extending at 50-65° C. for 0 to 1 s.

Methods for the rapid amplification of hepatitis B virus nucleic acid

The present application provides a magnetic bead-based nucleic acid extraction apparatus including a well plate, a magnetic attraction mechanism, a position moving mechanism, and an oscillation mechanism, a supporting base, and a fixing base disposed on the supporting base. The magnetic attraction mechanism includes a magnetic bar fixing base and a magnetic bar movably disposed on the magnetic bar fixing base. The position moving mechanism includes a ball screw and a driving motor; two ends of the ball screw are respectively connected to the magnetic bar fixing base and the driving motor. The oscillation mechanism includes a carrier and a main oscillation unit connected to the carrier. The main oscillation unit includes a motor spindle, an eccentric column, and a connecting base.

Magnetic bead-based nucleic acid extraction apparatus and use method

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Changsha, China

Xu Fan