Qingdao University

Xiyue Cao

Zonghua Wang

Jianfei Xia

Hua Sun

A electrochemical biosensor based on an aptamer/nano silver probe and an EXO I enzyme. Nano silver nanoparticles having the functions of identifying a target object and generating electrochemical signals and modified by an aptamer are used as a biological probe for detecting target biomolecules, under the initiation of the target object and the assistance of a complementary probe and the EXO I enzyme cyclic shear amplification, and by means of the DNA complementary pairing principle, the probe can be gathered on the surface of a gold electrode, the larger the concentration of the target biomolecules is, the larger the gathering degree of the induced aptamer/nano silver probe is, and meanwhile an EXO I exonuclease target cyclic amplification strategy is introduced in the identification process of the target object, so that the electrochemical biosensor can sensitively and efficiently detect target biological substances.

Growing community of inventors

Qingdao, China

Xiyue Cao