Chinese Academy of Sciences

Bgi-shenzhen Co., Limited

Bgi Shenzhen

Bgi Tech Solutions Co., Ltd

Bgi Genomics Co., Limited

Bgi Health Service Co., Ltd.

Bgi Diagnosis Co., Ltd.

Xiuqing Zhang

Huanming Yang

Fang Zhou Chen

Jun Wang

Yan Wang

Jian Wang

Hui Fu Jiang

Yong Qiu

Liansheng Huang

Shiying He

Xiaojiao Chen

Shengpei Chen

Chuan Li

Xuyang Yin

Xun Xu

Xu Han

Fei Gao

Xiaoyu Liu

Junwen Wang

Fang Chen

Huijuan Ge

Tao Chen

Zhaoling Xuan

Chunlei Zhang

Weiwei Xie

Tianbai Deng

Fuman Jiang

Zhenshang Wang

Huifei Chen

Xuchao Li

Xianghua Chai

Yuying Yuan

Mingzhi Ye

Hui Jiang

Zhongsheng Sun

Li Bao

Hanjie Wu

Zhongma Wang

Peng Fu

Xiaoyu Pan

Peipei Li

Shang Yi

Chunsheng Zhang

Lifu Liu

Furnan Jiang

Jing Zheng

Jingrong Lin

Disclosed is a synchronous and dynamic loading method in electro-magneto-thermo-mechanical multi-field coupling conditions. The method comprises the following steps: applying maximum pulse current to a test object by a pulse power supply to realize loading in extreme electric field and magnetic field conditions; meanwhile, generating a large amount of friction heat by the high-speed rotation of a rotating body and the friction of the test object to realize loading in an extreme-temperature field combined with a large amount of Joule heat and arc heat; synchronously applying pressure to the rotating body by a pressure device to realize loading of extreme force combined with the gravity of the rotating body and the friction force between the rotating body and the test object.

Synchronous and dynamic loading method in electro-magneto-thermo-mechanical multi-field coupling conditions

Disclosed is an electro-magneto-thermo-mechanical dynamic and synchronous loading device based on a wedge-shaped rotating body. The device comprises a carrier, a wedge-shaped rotating body and a pulse power supply, wherein the wedge-shaped rotating body is positioned above the carrier, the pulse power supply is connected to the carrier and the wedge-shaped rotating body through conductors, a test object is fixed on the carrier, the top of the wedge-shaped rotating body is connected to the output end of a driving shaft through a transmission shaft, the driving shaft drives the wedge-shaped rotating body to rotate and can apply downward pressure, and the wedge-shaped rotating body can be pressed against the test object and rotate on the surface of the test object.

Electro-magneto-thermo-mechanical dynamic and synchronous loading device based on wedge-shaped rotating body

A method for calculating a maximum output current of multiple thyristor converters connected in parallel, step: setting an operating time t; step: assuming a trigger angle; step: calculating a maximum output current of a single converter according to an output current model for the single converter; step: equally dividing a total output DC current into a plurality of parts according to a working duration of six converter bridge arms, thereby obtaining a pulse operating current of a single bridge arm; step: checking whether a present junction temperature of a thyristor is below a limiting temperature based on a thermal resistance model for the thyristor, if no, correcting the trigger angle, and repeating stepto stepuntil the condition is met; step: giving a present trigger angle; and step: giving a maximum output current of multiple converters connected in parallel.

Method for calculating maximum output current of multiple thyristor converters connected in parallel

Disclosed are a method and a system for determining genome copy number variation, which relates to the technical field of bioinformatics. The method and the system have clinical feasibility, and can precisely detect a micro-deletion/micro-duplication area of 0.5 M under the situation of using data of about 50 M.

Method and system for determining copy number variation

A method for detecting hydroxymethylation modification in nucleic acid comprises: glycosylating the nucleic acid, digesting with MspI, ligating the digested fragments to a biotin-labeled linker at both ends thereof, digesting with NlaIII; capturing the digested fragments using streptavidin magnetic beads to produce fragments having the biotin-labeled linker at one end and a CATG 4-base sticky end at the other end, wherein these fragments reveal modification information of their adjacent CCGG sites; ligating the CATG sticky end to a linker containing a recognition site of MmeI or Ecop15I, digesting with corresponding restriction endonuclease to produce short sequence fragments that can reveal modification information of their adjacent CCGG sites; and performing a tag number comparison to obtain information about methylation and hydroxymethylation modification relative levels. A use of the method is also provided.

Method for detecting hydroxylmethylation modification in nucleic acid and use thereof

The current invention is directed to methods for noninvasive detection of fetal genetic abnormalities by large-scale sequencing of nucleotides from maternal biological sample. Further provided are methods to remove GC bias from the sequencing results according to the difference in GC content of a chromosome. The current invention not only makes the detection much more accurate but also represents a comprehensive method for fetal aneuploidy detection including sex chromosome disorders such as XO, XXX, XXY, and XYY, etc.

Method for determining fetal chromosomal abnormality

The present invention provides a high-throughput sequencing method for methylated DNA, and use thereof. Particularly, the present invention provides a high-throughput sequencing method for methylated DNA, which combines methylated DNA immunoprecipitation, removal of repetitive sequences, and bisulfite treatment. The site of sequencing library will be decreased, and the cost will be reduced by using the method disclosed in the present invention.

High-throughput sequencing method for methylated DNA and use thereof

Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library.

Method for preparing nucleic acid library, its uses and kits

A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.

Method for single cell genome analysis and kit therefor

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Shenzhen, China

Xiuqing Zhang