Beijing Nano Hi-tech Material Co., Ltd.

Annoroad Gene Technology (beijing) Co., Ltd

Nanjing Annoroad Gene Technology Co. Ltd

Zhejiang Annoroad Bio-technology Co. Ltd.

Xiuli Wang

Yang Yu

Dawei Li

Gong Jin

Jiangping Tu

Zhaoling Xuan

Junbin Liang

Chongjian Chen

Wang Wang

Zhijiang Guo

Hongwei Chen

Ruilin Jing

The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3' end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.

Method for constructing next-generation sequencing library for detection of lowfrequency mutation and kit thereof

A method for treatment of saline-alkali land is provided, comprising at least one self-circulating process, the self-circulating process comprising: step 1, converting saline-alkali water deposited in the saline-alkali land into acidic water by an electrolysis system, whereby the salt content of the acidic water is lower than that of the saline-alkali water; and step 2, leaching the saline-alkali land with leaching water comprising the acidic water. A device for treatment of saline-alkali land according to the above-described method is also provided, comprising: an electrolysis system for converting saline-alkali water deposited in the saline-alkali land into acidic water, whereby the salt content of the acidic water is lower than that of the saline-alkali water; and a leaching system for leaching the saline-alkali land with leaching water comprising the acidic water.

Method and device for treatment of saline-alkali land

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Beijing, China

Xiuli Wang