The University of Texas System

Harvard College

Xiujun Li

George M Whitesides

Xinyu Liu

Maowei Dou

Wan Zhou

Sanjay Timilsina

Yu Sun

Katherine Andrea Mirica

Andres W Martinez

Scott T Phillips

Zhihong Nie

Chao-Min Cheng

Monica Mascareñas

Qijie Jin

Guanglei Fu

Martin M Thuo

Delfina Dominguez

Michael O'Brien

Gilberto Henao-Pabon

Frederique Deiss

A biosensing system configured to detect a target DNA and methods of detecting a target DNS are presented. The biosensing system comprises a biosensor comprising a suspension of a hybridization buffer containing dispersed gold nanoparticles, sodium chloride, and single-stranded DNA probes configured to undergo DNA hybridization with the target DNA.

Gold nanoparticle aggregation-induced quantitative photothermal biosensing using a thermometer

A method of synthesizing a smart paper transformer is provided. The method comprises combining paper with HAuCland stirring together in an aqueous solution to form a pulp. The pulp is treated with NaBHaqueous solution. The treated pulp is then washed and centrifuged with water a number of times to form a gold nanosponge (AuNS) catalyst pulp.

Smart paper transformer for enhanced catalytic efficiency and reusability of nanocatalysts

Embodiments described herein are related to a one-step surface modification method with 3-aminopropyl trimethoxysilane (APTMS), which was developed and applied for DNA immobilization on a paper-based device via the ionic adsorption.

One-step surface modification to graft DNA codes on paper and its bio-applications

Certain embodiments are directed to a paper hybrid microfluidic microplate. In certain aspects, the hybrid microfluidic microplate is a low-cost, sensitive, and fast diagnostic apparatus for detecting pathogens, diagnosing disease and other bio-applications, especially for low-resource settings.

Apparatuses and methods for pathogen detection using microfluidic biochips

Certain embodiments are directed to an ultrasensitive poly(methyl methacrylate) (PMMA) ELISA microfluidic microplate, where the protein is covalently bound to a poly-lysine modified or carboxylated PMMA surface.

Sensitive ELISA for disease diagnosis on surface modified poly(methyl methacrylate) (PMMA) microfluidic microplates

Certain embodiments are directed to paper and its hybrid microfluidic devices integrated with nucleic acid amplification for simple, cost-effective, rapid, and sensitive pathogen detection, especially in low-resource settings.

Methods and compositions for paper-based and hybrid microfluidic devices integrated with nucleic acid amplification for disease diagnosis

Certain embodiments are directed to sensors that enable the use of optimized biocompatible materials such as pre-anodized paper printed electrode transducer to detect binding of a target agent, wherein the surface is modified or functionalized through zero length cross-linker so that it interacts with or specifically binds a target such as sugars (glucose) or glycated proteins (HgbA1c).

Devices and methods using modified paper electrodes for the detection of hemoglobin A1C and glucose

Certain embodiments are directed to paper/polymer hybrid microfluidic devices integrated with nano-biosensors for pathogen detection and infectious disease diagnosis.

Methods and compositions for hybrid microfluidic devices integrated with nano-biosensors

Certain embodiments of the invention are directed to a photothermal immunoassay employing a thermometer or colorimetric detection method for sensitive quantitative readout based on the photothermal effect provided by a detection reagent.

Nanomaterial-based photothermal immunosensing for quantitative detection of disease biomarkers

MEMS devices fabricated using inexpensive substrate materials such as paper or fabric, are provided. Using paper as a substrate, low cost, simple to prepare, lightweight, disposable piezoresistive sensors, including accelerometers are prepared. Signal-processing circuitry can also be patterned on the substrate material. The sensors can be utilized as two-dimensional sensors, or the paper substrate material can be folded to arrange the sensors in a three dimensional conformation. For example, three sensors can be patterned on a paper substrate and folded into a cube such that the three sensors are orthogonally positioned on the faces of a cube, permitting simultaneous measurement of accelerations along three orthogonal directions (x-y-z). These paper-based sensors can be mass produced by incorporating highly developed technologies for automatic paper cutting, folding, and screen-printing. Also provided are methods of modifying paper for use as a substrate material in MEMS devices.

MEMS force sensors fabricated using paper substrates

The multiplexed electrochemical microfluidic paper-based analytical device comprises multiple detection zones for the detection of multiple biochemical analytes from one single sample. Cavity valves integrated on the device will deliver the sample to different detection zones. These analytes include, but are not limited to, urea, creatinine, creatine, glucose, lactate, ethanol, uric acid, cholesterol, pyruvate, creatinine, &#x3b2;-hydroxybutyrate, alanine aminotrasferase, aspartate aminotransferase, alkaline phosphatase, and acetylcholinesterase (or its inhibitors). This system will provide a simple and low-cost POC approach to obtain quantitative and multiple biological information from one sample (e.g. one drop of blood).

Microfluidic devices for multiplexed electrochemical detection

The disclosure provides low cost, portable three-dimensional devices for performing multiplexed assays. The devices comprise at least two substantially planar layers disposed in parallel planes, wherein one of the layers is movable relative to each other parallel to the planes to permit the establishment of fluid flow communication serially between the two layers.

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El Paso, TX, United States of America

Xiujun Li