Cibt America Inc.

Cathay Biotech Inc.

Cathay R&d Center Co., Ltd

Cathay Industrial Biotech Ltd.

Cathay (jinxiang) Biomaterial Co., Ltd.

Shanxi Synthetic Biology Institute Co., Ltd.

Cathay Biotec Inc.

Xiucai Liu

Howard Chou

Bingbing Qin

Naiqiang Li

Chen Yang

Charlie Liu

Min Xu

Yufeng Yang

Ling Chen

Wenbo Liu

Wenqiang Lu

Shuhua Zhang

Lijun Yu

Kai Wang

Yang Chen

Xiaoming Li

Yingli Hao

Zhenhua Pang

Duanfang Dai

Shanshi Guo

Benliang Hou

Wei Zhang

Yi Zheng

Jianglin Wang

Yunfeng Lei

Qinpei Li

Wenxiao Jiang

Shuya Min

Chaoxu Sun

Xiaochen Xu

Yuanbo Zhao

The present invention provides a process for producing a high temperature resistant polyamide, the high temperature resistant polyamide and the use thereof. The process comprises: optionally concentrating a polyamide salt solution, and then conducting the following operations: heating and pressurizing to a pressure of P1, and maintaining the pressure, wherein the temperature of the system is T1 at the end of the pressure-maintenance; depressurizing to a pressure of P2, wherein the temperature of the system is T2 at the end of the depressurization; and evacuating, whereby a polyamide melt is obtained; wherein P1 is 0.8-4 MPa, T1 is 250-290° C., T1<T2 and (T2−T1)/(P1−P2)=5-75. The process for producing a high temperature resistant polyamide according to the present invention is simple to operate and reduces energy consumption. The obtained high temperature resistant polyamide may be used as a raw material for such injection molded parts, molded articles, or fibers as high temperature resistant attachments for machines, automobiles, household appliances, toys, textiles, sporting goods, mobile phones, computers, laptops, GPS devices, or optical devices.

Method for producing high temperature-resistant polyamide, high temperature-resistant polyamide and use thereof

The present disclosure provides a polyamide 5× staple fiber, a preparation method and use thereof. The polyamide 5× staple fiber has a denier of 8.0-30.0D, a breaking strength of 2.0-6.0 cN/dtex, and an elongation at break of 30-100%. The polyamide 5× staple fiber has good mechanical properties and softness, and a blended wool yarn for manufacturing carpets with good mechanical properties, dyeability, and wear resistance can be obtained by using the polyamide 5× staple fiber.

Polyamide 5X staple fiber

Provided are astrain Am2525, with the preservation number thereof being CCTCC NO: M 2019419, and a method for producing long-chain dicarboxylic acids by means of fermenting the strain. The method for producing the long-chain dicarboxylic acids comprises preparing a seed solution by means of thestrain Am2525 and producing the long-chain dicarboxylic acids via fermentation of the seed solution. Compared with the parent, thestrain Am2525 has an enhanced resistance to the toxicity of a substrate decane, improves the productivity of long-chain dicarboxylic acids, reduces the cost of production, subsequent separation and purification are simple, and the fermentation production process is easy to implement on a large scale.

Strain for producing long-chain dicarboxylic acids and fermentation method therefor

This invention provides acid decarboxylase-prion subunit fusion polypeptides, nucleic acid sequences, expression vectors, and host cells expression such fusion polypeptides to produce various amino acids and derivatives of the amino acids such as polyamines.

Method of improving acid decarboxylase activity in vitro under alkaline pH

The present disclosure provides methods for isolating a long chain dicarboxylic acid such as a substantially pure or pure long chain dicarboxylic acid from a fermentation broth containing microbial cells.

Solid-solid separation process for long chain dicarboxylic acids

The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.

Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof

The present disclosure provides a system for refining long chain dicarboxylic acid, comprising: a first membrane filtration unit, for a first membrane filtration of a long chain dicarboxylic acid fermentation broth or a treated liquid therefrom; a first decolorization unit, for carrying out a first decolorization treatment to the filtrate obtained after the membrane filtration; a first acidification/crystallization unit, for carrying out a first acidification/crystallization of a filtrate obtained after the membrane filtration to give a solid-liquid mixture; a first separation unit, for a solid-liquid separation of the solid-liquid mixture; a drying unit, for drying the solid separated by the separation unit to give a first solid. By using the refining system according to the present disclosure, the purity of the obtained product is high, and the disadvantages such as poor quality of the product obtained by crystallization from a solvent and environment pollution caused by a solvent can be overcome.

Method and system for refining long chain dicarboxylic acid

The present disclosure provides a method for extracting long chain dicarboxylic acid, the method comprising: (1) subjecting a long chain dicarboxylic acid fermentation broth to a primary membrane filtration treatment to give a first filtrate; subjecting the first filtrate to decolorization, crystallization, and solid-liquid separation to give a first solid; (2) redissolving the first solid in water to form a solution; subjecting the solution to decolorization, crystallization by acidification, and solid-liquid separation to give a second solid. By the method, the resulted long chain dicarboxylic acid product has a high purity and no residual organic solvent.

Method and system for extracting long chain dicarboxylic acid

The present disclosure provides a system for extracting long chain dicarboxylic acid, the system comprising:

The present invention provides a decanedioic acid produced by microbial fermentation process, in which the content of C10 aliphatic acid and C10 hydroxy aliphatic acid is maintained at a very low level. The present invention also provides a preparation method of the decanedioic acid and a polymer prepared by using the decanedioic acid as monomer. The decanedioic acid provided by the present invention is prepared by microbial fermentation process. The decanedioic acid product which is produced through the processes of microbial fermentation and separation has a higher purity, a higher thermal stability, and a lower impurity content. The decanedioic acid provided by the present invention could satisfy the requirements of high grade product of polyamide or polyester to produce polymer with excellent qualities. The preparation method of the decanedioic acid provided by the present invention which has many advantages of mild reaction conditions, environmental friendliness, high yield, and good product quality, may replace the chemical method to be used in industrial scale production of the decanedioic acid.

Decanedioic acid produced by microbial fermentation process and preparation method thereof

Provided microorganisms genetically modified to overexpress an imine/enamine deaminase to enhance the production of lysine and lysine derivatives by the microorganism. Also provided a method of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.

Reducing the accumulation of imines/enamines for the production of amino acids or amino acid-derived products

The invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain. In particular, the invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution of CYP52A12 gene and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain.

Directed evolution of CYP52A12 gene and its use in dicarboxylic acid production

The invention provides CadA polypeptides with mutations that increase activity in alkaline pH compared to the wild-type lysine decarboxylase. The invention also provides methods of generating such mutant polypeptides, microorganisms genetically modified to overexpress the mutant polypeptides, and methods of generating such microorganism.

Modifications to lysine decarboxylase enzymes

The invention provides microorganisms genetically modified to overexpress biofilm dispersal related polypeptides to enhance the production of lysine and lysine derivatives by the microorganism, method of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.

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Shanghai, China

Xiucai Liu