Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Xiping Niu

Nicholas J Bate

Rebecca E Cahoon

Quinn Qun Zhu

Albert Laurence Lu

Saverio Carl Falco

Anthony John Kinney

J Antoni Rafalski

Timothy George Helentjaris

Zhongsen Li

James Kevin Presnail

Hajime Sakai

Edgar Benjamin Cahoon

Guo-Hua Miao

Yiwen Fang

Peizhong Zheng

Joan Tellefsen Odell

Xu Hu

Jian-Ming Lee

Sabine Susanne Hantke

Michele Morgante

Xuehua Hu

Clara Maria Alarcon

Nina Richtman

Molly Mary McMahon

Michaela Owens

Adane Kassa

Jonathan William Robeson

Bliss Marie Kernodle

Nicholas Bate

The present invention relates generally to methods of molecular biology and gene silencing to control pests.

Compositions and methods to control insect pests

Compositions and methods that allow for the rapid detection and accurate quantification of any polypeptides of interest are provided. Compositions include isolated polypeptides comprising at least one universal peptide tag, as well as isolated polynucleotides encoding such polypeptides. The universal peptide tags can be quantified by methods including, but not limited to, mass spectrometry, and can act as surrogates for determining the concentration of the polypeptides comprising the universal peptide tags. Methods provide for the detection and/or quantification of any polypeptides of interest that comprise at least one universal peptide tag, including methods using mass spectroscopy techniques. Methods are also provided for producing hosts, or cells or parts thereof, that comprise polypeptides comprising at least one universal peptide tag. Hosts, or cells, or parts thereof, include mammalian, bacterial, insect, yeast, viral or plant.

Universal peptide tags for transgene polypeptide analysis by mass spectrometry

The invention provides isolated pyruvate dehydrogenase kinase nucleic acids and their encoded polypeptides. The present invention provides methods and compositions relating to altering pyruvate dehydrogenase kinase levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

Polynucleotides encoding proteins involved in plant metabolism

Compositions and methods for modulating abscisic acid (ABA) perception and signal transduction in developing seed are provided. The methods and compositions find use in increasing yield in plants, particularly under abiotic stress. Compositions comprise genetic constructs known to affect ABA sensitivity, particularly ABA biosynthetic mutants and fragments and variants thereof.

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Methods and compositions for modulating plant development are provided. Polynucleotide sequences encoding ZmSNAC polypeptides are provided, as are the amino acid sequences of the encoded polypeptides. The sequences can be used in a variety of methods including modulating root development, modulating floral development, modulating leaf and/or shoot development, modulating senescence, modulating seed size and/or weight, and modulating tolerance of plants to abiotic stress. Transformed plants, plant cells, tissues, and seed are also provided. A stress-inducible ZmSNAC1 promoter is also provided.

Maize stress-responsive promoter

The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for regulatory elements providing expression preferentially in the inflorescence meristem and developing floral tissues. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided, as well as expression constructs, vectors, and transformed cells and plants.

Early-inflorescence-preferred regulatory elements and uses thereof

Compositions and methods for modulating gene expression in plants are provided. The compositions comprise novel genes and promoters as well as regulatory and binding elements therein. The methods comprise the modulation of gene expression using the compositions of the invention. Compositions and methods are provided to reduce the impact on plants of biotic and abiotic environmental stresses. Accordingly, the compositions and methods may also be used to decrease the necessary amounts of conventional stress reduction treatment as well as to increase plant yield.

Genes and regulatory DNA sequences associated with stress-related gene expression in plants and methods of using the same

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Johnston, IA, United States of America

Xiping Niu