Applera Corporation

Applied Biosystems, LLC

Other

Life Technologies Corporation

Dh Technologies Development Pte. Ltd.

Pe Corporation

Xiongwei Yan

Pau Miau Yuan

Scott C Benson

Khairuzzaman Bashar Mullah

Brian Lee Evans

Sheri Miraglia

Subhasish Purkayastha

Subhakar Dey

Darryl J C Pappin

Krishna G Upadhya

Sasi K Pillai

Joe Y Lam

Pau-Miau Yuan

Kuo-Liang Hsi

Sheri Miragila

Sylvia W Yuen

Helena Huang

Chu-An Chang

The present invention is directed to fluorescent rhodamine dyes having spectral properties suited to the creation of multiplex assay systems for use in molecular biology, cell biology and molecular genetics. The rhodamine dyes have the following structure.

Modified rhodamine dye and use thereof in biological assays

Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

Quencher and reporter dye combinations

Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.

Peptide conjugates and fluorescence detection methods for intracellular caspase assay

This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.

Mass tags for quantitative analyses

Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.

Sulfonated [8,9]benzophenoxazine dyes and the use of their labelled conjugates

Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates

Fluorescent, sulfonated 3,7-diamino-&lsqb;8,9&rsqb;benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.

Sulfonated &lsqb;8,9&rsqb; benzophenoxazine dyes and the use of their labelled conjugates

A new class of red-emitting, fluorescent [8,9]benzophenoxazine dyes are provided that are useful for staining nucleic acids in a variety of contexts, including in solutions, in electrophoretic gels or other matrices, in blotting experiments and in assays employing intact, live cells. The new dyes are brighter and permeate cells faster than currently available red-emitting live-cell nucleic acid stains.

Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for

Growing community of inventors

Dublin, CA, United States of America

Xiongwei Yan