Jiangnan University

Taixing Dongsheng Bio-tech Co., Ltd

Xinrui Zhao

Jian Chen

Guocheng Du

Jianghua Li

Jingwen Zhou

Wei Lu

Yuan Qian

Bohan Zhang

Fei Yu

The disclosure discloses a genetically engineered strain for producing porcine myoglobin and fermentation and purification thereof, and belongs to the technical field of genetic engineering. The disclosure realizes efficient secretion and expression of porcine myoglobin by integrating the gene of porcine myoglobin in. On this basis, optimization of the medium and culture conditions of recombinantcan significantly increase the titer of porcine myoglobin, so that the titer can reach 285.42 mg/L under fermenter conditions. In addition, by creatively adding different concentrations of ammonium sulfate to fermentation broth step by step, the purity of myoglobin obtained by final concentration is up to 88.0%, and the purification rate is up to 66.1%. The disclosure realizes efficient expression and high purification of porcine myoglobin from various steps such as synthesis, fermentation and purification of porcine myoglobin, and provides broad prospects for industrial production of porcine myoglobin.

Genetically engineered strain for producing porcine myoglobin and food-grade fermentation and purification thereof

Growing community of inventors

Wuxi, China

Xinrui Zhao