Chinese Academy of Sciences

Xinna Zhu

Xueli Zhang

Hongtao Xu

Yanhe Ma

Jing Ran Chen

Jinlei Tang

Zaigao Tan

Pingping Liu

The invention relates to the field of producing succinate byfermentation. Specifically, the invention provides an engineered recombinantfor producing succinate, wherein saidcontains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinantfor producing succinate, and a method of using the engineered recombinantfor producing succinate.

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Provided is recombinantcomprising a mutated ldhA gene. Disclosed are use of the recombinantin the preparation of D-lactate, and method of preparing D-lactate by employing the recombinant. In the method of preparing D-lactate, fermentation can be conducted at a high temperature (42-50&#xb0; C.), and aqueous ammonia or sodium hydroxide can be used as a neutralizer during the fermentation.

The invention relates to the field of modifyingthrough genetic engineering. Specifically, the invention provides ancontaining a mutated lpdA gene. The invention also relates to use of thein the production of chemical material such as ethanol, and succinate etc. The invention also provides a method of producing chemical materials such as ethanol and succinate etc. by using the, as well as a method for increasing the activity of pyruvate dehydrogenase inby introducing a mutated lpdA gene.

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Tianjin, China

Xinna Zhu