Zhejiang University

Xingqian Ye

Donghong Liu

Shiguo Chen

Huan Cheng

Jianwei Zhou

Hua Zhang

Tian Ding

Enbo Xu

Weiwei Hu

Jiaqi Zheng

Hao Wang

Jiayun Xu

Lufeng Yan

Danli Wang

Jianle Chen

Junhui Li

Chantapakul Thunthacha

A method for promoting the solubility of rhamnogalacturonan I (RG-I) pectin based on polysaccharide interaction is provided. The method includes the following steps: adding an aqueous solution of negatively charged polysaccharides to an RG-I pectin extract, mixing uniformly, adjusting the potential of Hydrogen (pH) of the compound to 3-7, performing ethanol precipitation to obtain a precipitate; washing the precipitate, followed by drying and crushing to obtain a polysaccharide-pectin compound; where the RG-I pectin extract is obtained by extracting orange peel powder with dilute hydrochloric acid.

Method for promoting solubility of RG-I pectin based on polysaccharide interaction

A preparation method for a nanopore starch-based adsorbent. The method mainly comprises: constructing a large number of lamellar crystals on surfaces of starch granules, and inducing the formation of a nano-scale pore channel structure by means of the lamellar crystals. A large number of control tests have proved that the flaky crystals have direct influence on the formation of the nano-scale pore channel structure. The construction of a pore channel structure in a starch matrix material greatly increases the specific surface area, and improves the adsorption properties. In addition, the method is beneficial to the introduction of functional particles (magnetic particles), and avoids the problem of pore channel blockage due to first forming pores and then introducing functional particles.

Starch-based adsorbent with nanopores and preparation method thereof

The present invention discloses an ultrasound-electrode-nano-porous membrane coupling hydrogen production and sterilization system, which comprises a vessel and a plurality of hydrogen production units arranged in the vessel. The hydrogen production unit comprises a cavity, and a broadband ultrasonic generator and a circular hydrogen production electrode located in the cavity. The circular hydrogen production electrode wraps around the broadband ultrasonic generator. A bottom membrane at the bottom of the cavity is hydrophobic at the inner side and hydrophilic at the outer side, and a side membrane of the cavity is hydrophilic at the inner side and hydrophobic at the outer side and is mounted vertically or obliquely upwards by an angle of 0-45 degrees. The system has the functions of continuously and efficiently facilitating self-circulation micro-flow of a liquid system, hydrogen dissolution (as nano-bubbles), conversion from the interface to the bulk phase and dispersion of bubbles, sterilization and others.

Ultrasound-electrode-nano-porous membrane coupling hydrogen production and sterilization system

The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.

Ultrasound and pressure assisted method for extracting pectin rich in RG-I

The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40&#xb0; C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.

Ultrasonic-assisted method of extracting a pectin rich in RG-I

The present disclosure discloses a method for extracting a RG-I-rich pectic polysaccharide. The method comprises the following steps: mixing and stirring a citrus fruit peel powder and a dilute hydrochloric acid solution at a low temperature to destroy cell walls of citrus peels, filtering, and then mixing the retentate with a sodium hydroxide solution for low temperature extraction, adding ethanol to the solution obtained by filtering to precipitate, and washing the obtained polysaccharide with ethanol and drying to obtain the above pectic polysaccharide. Compared with the traditional extraction method, this method reduces the hydrolysis of pectin side chains by controlling the extraction temperature, and the extracted pectic polysaccharide is rich in RG-I domain and has higher biological activity such as cancer and cardiovascular disease prevention. Energy can be greatly saved because of the low extraction temperature. The extracted pectic polysaccharide can be potentially used as functional food ingredients.

Method for extracting RG-I-rich pectin

The present invention discloses a mixture of fucosylated chondroitin sulfate oligosaccharides having an average molecular weight between 2700 Da and 3600 Da, in which 90% of total components has a molecular weight between 1800 Da and 5400 Da. The mixture of fucosylated chondroitin sulfate oligosaccharide of the present invention has very low anti-FIIa factor activity, very low anti-FXa factor activity, very high anti-FXase factor activity, i.e., having a significant activity in selectively inhibiting intrinsic FXase, and a significant antithrombotic activity and no risk of bleeding and hypotension that are proved through animal experiments. The present invention also discloses a method for rapidly producing fucosylated chondroitin sulfate oligosaccharides by using sea cucumber as raw material, and the method includes: intercepting and concentrating crude polysaccharide with a membrane, deacetylation with hydrazine, pyrolysis with nitrous acid, reduction with sodium borohydride, membrane separation, etc., achieving rapid preparation and high yield.

Mixture of fucosylated chondroitin sulfate oligosaccharides and method for rapidly producing the same

The present disclosure discloses a method for preparing a citrus-derived complex prebiotic agent. The method comprises the following steps: removing external citrus peels after blanching citrus fruits with hot water, soaking citrus segments after splitting with an acid solution and an alkali solution to remove membranes of the citrus segments, and adjusting the pH of the obtained alkali soaking water with acid soaking water or acid to be weakly acidic or neutral to obtain the complex prebiotic agent, which has the function of reversing intestinal flora disorders. Compared with the traditional prebiotic product preparing method, the product prepared by the method contains prebiotic functional components with different targets such as pectin, flavonoids, oligosaccharides, and other fibers at the same time, and has the efficacies of regulating intestinal flora and reversing intestinal flora disorders caused by antibiotics. At the same time, the preparing method has the characteristics of simple operation and low cost, perfectly supplements a comprehensive utilization process of citrus fruit processing, solves the discharge problem of citrus fruit processing water, and generates very good social benefits.

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Hangzhou, China

Xingqian Ye