Jiangnan University

Xingfei Li

Jie Long

Zhengyu Jin

Yaoqi Tian

Xing Zhou

Long Chen

Zhengjun Xie

Yuxiang Bai

Chao Qiu

Yufei Hua

Caimeng Zhang

Shunuan Yang

Yeming Chen

Xiangzhen Kong

Ziying Ye

Cheng Lu

Disclosed is a method for purifying pea albumin PA1a by using a negatively charged polysaccharide, which belongs to the field of protein extraction technology. According to the invention, with a natural edible negatively charged polysaccharide as a recycling material, based on the technical principle that the negatively charged polysaccharide preferentially binds to non-target proteins to precipitate and undergoes phase separation relative to the target protein, the separation and purification of pea albumin PA1a are realized to prepare pure PA1a. From the perspective of resource recycling, the invention can realize high value-added utilization of byproducts of pea protein isolate processing and prepare high-purity high-sulfur active protein components therefrom, thereby providing technical support for subsequent research. The invention features improved comprehensive utilization of pea whey, high recycle rate of PA1a protein with high purity, low requirements on equipment, simple and rapid operation and no environmental pollution.

Method for purifying pea albumin PA1a by using negatively charged polysaccharide

Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86site, the 373site, the 374site, the 496site, the 507site, or the 747site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Agarase mutant with improved thermal stability and application thereof

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Wuxi, China

Xingfei Li