Bgi Genomics Co., Limited

Bgi Diagnosis Co., Ltd.

Xiaoyu Pan

Jun Wang

Jian Wang

Hui Fu Jiang

Jing Guo

Fan Fan

Fang Zhou Chen

Xiuqing Zhang

Huanming Yang

Shida Zhu

Fang Chen

Shengpei Chen

Xuyang Yin

Chunlei Zhang

Weiwei Xie

Hui Jiang

Jialou Zhu

Chunsheng Zhang

The present invention provides a method for determining the nucleic acid composition in a total nucleic acid mixture comprising a first nucleic acid and a second nucleic acid. The method comprises: 1) treating the total nucleic acid mixture with a bisulfate, to convert the non-methylated cytosine in the total nucleic acid mixture into uracil, and obtain a converted total nucleic acid mixture; 2) subjecting the converted total nucleic acid mixture to multiplexed fluorescent quantitative PCR using a first set of amplification primers and a second set of amplification primers; and 3) based on the ratio R of the methylated amplification product to the non-methylated amplification product of the predetermined nucleic acid fragment, a methylation proportion Mof the predetermined nucleic acid fragment in the first nucleic acid, and a methylation proportion Mof the predetermined nucleic acid fragment in the second nucleic acid, determining the nucleic acid composition in the total nucleic acid mixture.

Method for determining nucleic acid composition of nucleic acid mixture

Growing community of inventors

Shenzhen, China

Xiaoyu Pan