Roche Molecular Systems, Inc.

Xiaoying Chen

Wei-min Liu

Yan Li

Xiaoju (Max) Ma

Sim-Jasmine Truong

Thomas W Myers

Nancy J Schoenbrunner

Nancy Patten

Sim C Truong

Suzanne Cheng

Accurate and fast mapping of sequencing reads obtained from a targeted sequencing procedure can be provided. Once a target region is selected, alternate regions of the genome that are sufficiently similar to the target region can be identified. If a sequencing read is more similar to the target region than to an alternate region, then the read can be determined as aligning to the target region. The reads aligning to the target region can then be analyzed to determine whether a mutation exists in the target region. Accordingly, a sequencing read can be compared to the target region and the corresponding alternate regions, and not to the entire genome, thereby providing computational efficiency.

Accurate and fast mapping of reads to genome

The invention comprises suppressor oligonucleotides for reducing amplification of a non-target nucleic acid sequences; the method of designing and using such oligonucleotides, as well as kits and reaction mixtures.

Suppression of non-specific amplification with high-homology oligonucleotides

Growing community of inventors

Alameda, CA, United States of America

Xiaoying Chen