Wuhan Yzy Biopharma Co., Ltd.

Wuhhan Kotel Big Date Corporation

Hubei Biopesticide Engineering Research Center

Xiaoyan Liu

Wei Chen

Jing Zhang

Pengfei Zhou

Lei Zhu

Yan Gong

Rui Wang

Cheng Zhang

Ronghua Zhou

Ben Rao

Xianqing Liao

Lingli Hu

Xiang Zhou

Yong Min

Liqiao Shi

Ling Chen

Yimin Qiu

Jingzhong Yang

Hui Zhong

Sisi Fu

Fen Liu

Zhigang Cao

A method and system for constructing a transverse topological relationship and a memory are provided. In the method, lane group data in a high-definition map is acquired; for each lane group, a shared boundary line group of two adjacent lanes is sequentially extracted, and the number of parallel boundary line elements is determined; if the number is 1, a transverse topological relationship between the two adjacent lanes is not generated, otherwise the number and types of boundary line units on the parallel boundary line elements are determined; and if the number of the boundary line units is 1, the transverse topological relationship between the two adjacent lanes is generated, otherwise segmentation processing is performed on the lane group along a lane direction, and the transverse topological relationship between two adjacent lanes in each of segments, which are obtained by the segmentation processing on the lane group, is sequentially generated.

Method, system and memory for constructing transverse topological relationship of lanes in high-definition map

Provided are compositions and methods for preparing a cell suitable for producing a bispecific antibody. A plurality of eukaryotic cells are incubated with an agent under conditions to allow the cells to arrest at G1/S phase. The agent is then removed from the cells and the cells are transfected with a first vector comprising a sequence encoding a first monovalent antigen-binding unit having specificity to a first antigen and a second vector comprising a sequence encoding a second monovalent antigen-binding unit having specificity to a second antigen. A cell is identified from the plurality of cells that expresses both the first and the second antigen-binding units.

Preparation and selection of cells for producing bispecific antibodies

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Wuhan, China

Xiaoyan Liu