Nanjingjinsirui Science & Technology Biology Corporation

Xiaowu Liu

Zhuying Wang

Fang Liang Zhang

Luquan Wang

Chunjiao Wang

Zhibing Liu

Yun He

Tianhui Xia

Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described.

Methods and compositions for enhanced expression and secretion of proteins

An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5'-UTR, ORF and 3&#x2032;-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm

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Nanjing, China

Xiaowu Liu