Osel, Inc.

Geneweave Biosciences, Inc.

Xiaowen Liu

Qiang Xu

John A Lewicki

Diego Ariel Rey

David A Simpson

Chia-Hwa Chang

Marc Rehfuss

Kirsten Essenmacher

Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.

Non-replicative transduction particles and transduction particle-based reporter systems

Methods and compositions for targeting heterologous polypeptides to bacterial cell walls are provided.

Surface expression of biologically active proteins in bacteria

This disclosure relates to a modified version of cyanovirin-N protein and a transformedbacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformedbacterium. In addition, this disclosure describes two novel promoters originated from, which are capable of directing a high level of gene expression inbacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modifiedbacterium containing the expression cassette, a method for recombinant gene expression inbacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.

Modified cyanovirin-n polypeptide

Growing community of inventors

Cupertino, CA, United States of America

Xiaowen Liu