Nanjing Agricultural University

Xiaosan Huang

Zhihua Xie

Shaoling Zhang

Qian Liu

Peng Wang

Caihua Xing

Linlin Xu

Huijun Jiao

Qinghai Qiao

Kaijie Qi

Huizhen Dong

Likun Lin

Juyou Wu

Yaojun Chang

A gene PpHSP21 with black spot disease resistance is isolated from. A nucleotide sequence of the gene PpHSP21 is shown in SEQ ID NO. 1. An amino acid sequence of an encoded protein of the PpHSP21 gene is shown in SEQ ID NO. 2. By constructing the plant overexpression vector and silencing vector of the gene PpHSP21, the gene PpHSP21 is introduced into the plant by the-mediated genetic transformation method, so that the gene PpHSP21 is able to be overexpressed in the plants, thereby significantly improving black spot disease resistance in plants. The discovery and identification of the gene PpHSP21 provide new genetic resources for stress resistance molecular design and breeding in plants, and to provide new genetic resources for the implementation of green agriculture. The development and utilization of the genetic resources is conducive to reducing agricultural production costs and achieving environmental friendliness.

Genewith black spot disease resistance inand its applications in improving black spot disease resistance in plants

Some embodiments of the disclosure provide a protein for promoting the growth of a pollen tube and a gene PbrTTS1 encoding the protein. The amino acid sequence of the protein is shown in SEQ ID No: 1. The nucleotide sequence of the gene PbrTTS1 encoding the protein is shown in SEQ ID No: 2. Other embodiments of the disclosure provide a primer for amplifying the gene PbrTTS1 encoding the protein. The primer includes a forward primer and a reverse primer. The nucleotide sequence of the forward primer is shown in SEQ ID No: 3, and the nucleotide sequence of the reverse primer is shown in SEQ ID No: 4.

Protein of 'dangshan suli' having function of promoting growth of pollen tube, encoding gene PBRTTS1 and use thereof

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Nanjing, China

Xiaosan Huang