Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

University of Missouri

University of Bologna

Xiaomu Niu

Dwight T Tomes

Haiyin Wang

Sabine Susanne Hantke

Stephen M Allen

Hajime Sakai

Kanwarpal Singh Dhugga

Deping Xu

Graziana Taramino

Timothy George Helentjaris

Lu Liu

Bailin Li

Howard P Hershey

Stanley Luck

Scott V Tingey

Dilbag Singh Multani

Victor Llaca

Edward Bruggemann

Norbert Brugière

Giorgio Sponza

Shane E Abbitt

Robert B Meeley

Jeffrey Habben

Dale F Loussaert

Kevin A Fengler

Michele Morgante

Bruce J Drummond

Shuping Jiao

Roberto Tuberosa

Silvio Salvi

Daniel M Goldman

Mary A Rupe

Chun Ping Li

Jeffrey R Schussler

Norbert Brugiere

Mei Guo

Chris Zinselmeier

Odd-Arne Olsen

Mai Komatsu

Igor Cunha Oliveira

Jeffrey E Habben

Hajime Saki

Michele University Of Udine Morgante

S Dept Agroenvironmental Sci And Techn Salvi

G Dept Agroenvironmental Sci And Techn Sponza

R Dept Agroenvironmental Sci And Tech Tuberosa

Methods for modulating plants using optimized ZmME293 down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear number and/or reduction in time to scenescence.

Down-regulation of a homeodomain-leucine zipper I-class homeobox gene for improved plant performance

The present invention provides a method, apparatus, and system for efficient, high throughput, consistent cleaning of soil from the root system of a plant. In one embodiment, the method generally comprises positioning a plant adjacent a sprayer assembly having at least one nozzle angled so as to impact the root system at a predetermined position, providing a stream of water from a reservoir through the nozzle of the sprayer assembly, and rotating at least one of the root system and the nozzle relative to the other to remove the soil from the root system. The present invention further describes a recycling system adapted to recycle water expended during the cleaning process to minimize losses and to capture and return dislodged soil to the field from which the plant is extracted.

High throughput root washing method, apparatus, and system

Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.

Plants with altered root architecture, related constructs and methods involving genes encoding exostosin family polypeptides and homologs thereof

Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (LLRK) polypeptides and homologs thereof

This invention relates to isolated nucleic acid fragments encoding high affinity nitrate transport components. The invention also relates to the construction of recombinant DNA constructs encoding all or a portion of nitrate transport components, in sense or antisense orientation, wherein expression of the recombinant DNA construct may alter levels of the nitrate transport components in a transformed host cell.

Nitrate transport components

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a root-preferred promoter and terminator isolated from the maize MSY coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Root-preferred regulatory elements

The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence-based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.

Methods and compositions for modulating flowering and maturity in plants

This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes, particularly in plant seeds and related female reproductive tissue. Compositions comprise novel nucleotide sequences for a seed-preferred promoter known as eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Early endosperm promoter eep2

The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions and methods include expression cassettes and transformed plants and provide for downregulation of cytokinin oxidase in a plant.

Cytokinin oxidase promoter from maize

The present disclosure is directed to isolated nucleic acid fragments comprising a root-preferred maize NAS2 promoter, a functional fragment thereof, and end-uses and methods utilizing said fragments. Functional fragment NAS2 promoters include those containing the Root Motif 1, the CAAT box, and the TATA box; the Pyrimidine Box, the Pyrimidine-rich Stretch, the QAR, the Root Motif 1, the CAAT box, and the TATA box; and the Pyrimidine Box, the Pyrimidine-rich Stretch, the QAR, the Root Motif 1, and the CAAT box.

Plants with altered root architecture, involving the rum1 gene, related constructs and methods

The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.

Methods for large scale functional evaluation of nucleotide sequences in plants

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a root-preferred promoter and terminator isolated from the maize 6PGD coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.

The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.

Brachytic2 (Br2) promoter from maize and methods of use

Growing community of inventors

Johnston, IA, United States of America

Xiaomu Niu