Kyoto Daiichi Kagaku Co., Ltd.

Xiaoming Dou

Harumi Uenoyama

Yoshinori Yamaguchi

Yung Xiang Wang

Masayuki Yagi

Toshio Takama

Yukihiro Ozaki

Hiroshi Yamamoto

Yutaka Yamasaki

Masao Kono

Koji Matsuoka

Kentaro Shimada

A total reflection cell has a total reflection prism on at least one surface thereof. A mixed solution containing a gold colloid labelled antibody which is adsorbed on a gold colloid is stored in the total reflection cell, and a sample solution containing an antigen causing antigen-antibody reaction with the antibody is added thereto for forming a gold colloid labelled immune complex. A measuring beam is introduced into the total reflection prism from an incident optical system at an angle &#x3b8; of incidence causing total reflection and an outgoing beam from the total reflection prism is received by a measuring optical system, thereby measuring absorption by the gold colloid labelled immune complex and carrying out qualification and determination of the antigen.

Apparatus for immune analysis

A method for measuring the concentration of polynucleotide in a sample comprising the steps of: (1) preparing a sample, a Raman scattering active agent capable of bonding to a polynucleotide and a surface-enhanced Raman scattering (SERS) active substrate capable of capturing the agent; (2) mixing the sample, the agent and the substrate to capture the agent which has not been bonded to polynucleotide on the surface of the substrate; (3) irradiating the mixture; and (4) measuring the SERS radiation which is generated by the agent captured on the surface of the substrate.

Method for measuring the concentration of polynucleotides

An excitation beam from a laser is passed through a bandpass filter, and applied to a sample by a collimator lens. An optical adjusting part comprising a camera lens and a converging lens is provided for receiving scattered light from the sample, so that the camera lens receives the scattered light and converts the same to a parallel beam. The converging lens has chromatic aberration, and receives and converges the parallel beam from the camera lens. An inlet slit of a spectroscope is provided as an inlet port on a converging position by the converging lens. This inlet slit is a circular hole having a diameter of about 200 .mu.m. The inlet slit is arranged on a position for converging anti-Stokes-Raman scattered light through the chromatic aberration of the converging lens.

Measuring method and measuring apparatus by light scattering

In order to make a Raman spectral measuring apparatus effective for measurement of a vital sample and attain miniaturization and weight reduction, a near infrared laser diode is employed as a light source, and a spectroscope for receiving Raman scattered light from the sample is formed by a polychrometer comprising a concave diffraction grating and a multichannel photodiode array. The Raman scattered light incident through an inlet slit is separated into its spectral components by the diffraction grating, and its spectra are simultaneously detected by the photodiode array over a prescribed wavelength region.

Spectral measuring apparatus and automatic analyzer

A concave urine collector is set under a front end portion of a stool body, so that urine can be collected in a bowl. Single fiber holes are set on a front end and a left side of the urine collector respectively, while condenser lenses for condensing light on the bowl side, an excitation light fiber member carrying excitation light from an excitation light source and a Raman light receiving fiber member for receiving Raman light are set in the fiber holes respectively. The excitation light fiber member is connected with the excitation light source, and the Raman light receiving fiber member communicates with a spectro-detector. A Raman signal separated into its spectral components detected by the spectro-detector is data-processed by a data processing part, and outputted from a data output part as a measurement result.

Urogenous component measuring apparatus for qualitatively/quantitatively

Acid is added to a target sample such as urine, blood plasma or serum for converting mevalonic acid contained in the sample to lactone mevalonate, and the sample is irradiated with near infrared Raman excitation light, for determining mevalonic acid by measuring generated Raman scattered light. This method employs no mediatory reaction with simple pretreatment, requires no high-priced measuring apparatus, and can determine mevalonic acid in a short time.

Optical measuring method for mevalonic acid

A sample solution containing protein is irradiated with excitation light of a single wavelength which is emitted from a light source, so that light scattered from the sample solution is received and separated into its spectral components in a spectroscope, thereby obtaining light scattering spectra. Protein is quantitatively measured through intensity of a light scattering spectrum in a shift wavenumber of 100 to 3100 cm.sup.-1 with respect to the excitation wavelength among the light scattering spectra or an integral value in a proper range therein. As to a body fluid sample, the sample is irradiated with excitation light and Raman scattering spectral intensity values are measured at a plurality of wavenumbers in an arbitrary wavenumber range, and a plurality of components in the sample are analyzed simultaneously by multivariate regression analysis.

Method of optically measuring component in solution

As to respective expiration components to be measured, wavelengths having excellent correlations between component concentrations and Raman spectral intensity values are previously selected as measuring wavelengths which are specific to the components, an expiration specimen is irradiated with Raman excitation light, Raman spectra at the measuring wavelength which is specific to nitrogen and those at the measuring wavelengths previously selected for the components to be measured respectively are measured, Raman spectral intensity ratios of the components to the Raman spectral intensity of nitrogen are obtained, and the respective expiration components are quantitatively analyzed through a calibration curve which is previously prepared as to the Raman spectral intensity ratios of the respective components to nitrogen and concentrations. It is possible to provide a measuring method utilizing Raman spectroscopy, which can directly determine intra- expiratory components in a short time with no requirement for expendable items.

Optical measuring method of expiration components

A Raman scattered light measuring apparatus comprises a near infrared semiconductor laser diode as a light source for irradiating a sample with excitation light, while a photoreceiving part for receiving Raman scattered light from the sample comprises a bandpass filter having a vibration wavenumber which is specific to a sample component to be measured as a central wavelength of its transmission region, and a detector consisting of a photodiode of Ge, InAs or InGaAs or a photomultiplier having sensitivity in a near infrared region for detecting Raman scattered light which is transmitted through the bandpass filter.

Raman scattered light measuring apparatus

A total reflection cell has a total reflection prism on at least one surface thereof. A mixed solution containing a gold colloid labelled antibody which is adsorbed on a gold colloid is stored in the total reflection cell, and a sample solution containing an antigen causing antigen-antibody reaction with the antibody is added thereto for forming a gold colloid labelled immune complex. A measuring beam is introduced into the total reflection prism from an incident optical system at an angle .theta. of incidence causing total reflection and an outgoing beam from the total reflection prism is received by a measuring optical system, thereby measuring absorption by the gold colloid labelled immune complex and carrying out qualification and determination of the antigen.

Method of and apparatus for immune analysis

As to reaction solutions in which the amount of each potassium ferricyanate, glucose oxidase and glucose substrate are set at a constant value while glucose concentrations are varied, intensity changes of peaks at a shift wavenumber of 2081 cm.sup.-1 in light scattering spectra are measured every 10 seconds, the maximum values of the intensity changes of the peaks at the respective glucose concentrations obtained, for obtaining a calibration curve from correlations between the maximum values of the reaction velocities and the glucose concentrations. The calibration curve is employed for carrying out quantitative measurement of an unknown sample.

Method of measuring enzyme reaction by Raman scattering

In order to quantitatively measure an Amadori compound by a simple optical method utilizing light scattering, a sample containing an Amadori compound which is stored in a cell is irradiated with excitation light from an He--Ne laser unit so that scattered light from the sample is received and separated into its spectral components for obtaining a light scattering spectrum, and a light scattering peak existing at 820 to 840 cm.sup.-1, 1655 to 1660 cm.sup.-1, 2000 to 2020 cm.sup.-1, 2080 to 2100 cm.sup.-1, 2460 to 2470 cm.sup.-1 or 2530 to 2600 cm.sup.-1 in shift wavenumber with respect to the excitation wavelength in the light scattering spectrum is detected by a detector. The saccharide concentration or the saccharification ratio of the Amadori compound is measured by a calibration curve through the peak intensity or the peak integral value of the light scattering peak.

Method of measuring Amadori compound by light scattering

Excitation light from a light source is divided into a sample beam and a correction beam by a half mirror, so that the sample beam is converged on a sample in a sample part by convergent lenses. Condenser lenses are provided in order to converge scattered light from the sample on an inlet slit of a spectroscope, and a holographic notch filter which is set to include the wavelength of the excitation light in its notch region is arranged in order to remove the same wavelength component as the excitation light from the scattered light for selecting target light. The target light and the correction beam are guided onto the same optical axis by a half mirror, to be incident upon a polychrometer through the inlet slit and simultaneously detected. The detected value of the target light is corrected by a simultaneously detected intensity of the correction beam.

Spectral measuring method and spectral measuring apparatus

An apparatus and a method for measuring concentrations of components with light scattering allows measurement of concentrations of components in a measuring object to be accomplished in non-destructive fashion by using light in near-infrared wavelength ranges with good transmissivity and relatively low light quantum energy. The apparatus is composed of a light irradiator (1) for irradiating excited light in a near-infrared wavelength range to a measuring object (16), a photodetector (2) for receiving and spectrally separating Raman scattered light derived from the measuring object (16), and an arithmetic unit (3) for calculating concentration of a component in the measuring object (16) from intensity of the Raman scattered light and outputting a calculation result.

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Kyoto, Japan

Xiaoming Dou