China Agricultural University

Beijing University of Agriculture

Xiaolong Qi

Yu Chen

Yu Zhong

Chenxu Liu

Di Liu

Yong Guo

Shaojiang Chen

Xiangguo Wang

Baojian Chen

Mengran Li

Zongkai Liu

Hemin Ni

Yanyan Jiao

Provided are a parthenogenetic haploid induction gene DMP and an application thereof. The parthenogenetic haploid induction genes AtDMP8 and AtDMP9 are cloned from. Experiments have shown that mutations of AtDMP8 and AtDMP9 can produce parthenogenetic haploid inducibility, to enable dicotyledonous crops to be induced to produce haploids via parthenogenetic means. The present invention was further verified in tomatoes, and it was also found in tomatoes that the mutation of SlDMP can produce parthenogenetic haploid inducibility. The invention lays an important foundation for broadening the application of haploid breeding technology on dicotyledonous plants and revealing the biological mechanism of parthenogenetic haploid production. Given the universality of the utilization of haploid breeding technology in the current breeding industry, the invention has very wide application space and market prospects.

Parthenogenetic haploid induction geneand application thereof

The present invention belongs to the technical field of embryo cryopreservation. In particular, the present invention relates to an in-vitro embryonic culture medium for improving the thawed recovery rate of an embryo, comprising an inhibitor of Cathepsin L inhibitor, for example, E-64d. The present invention also relates to a method for improving the freezing resistance and the thawing recovery rate of an embryo and a method for freezing an embryo. With the present invention, the freezing resistance and the thawing recovery rate of an embryo are significantly improved.

In-vitro embryonic culture medium comprising an inhibitor of cathepsin L and the use of the same in embryo cryopreservation

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Beijing, China

Xiaolong Qi