Illumina Cambridge Limited

Illumina Corporation

Xiaolin Wu

Xiaohai Liu

John Milton

Colin Lloyd Barnes

Geoffrey Paul Smith

Silke Ruediger

Nikolai Nikolaevich Romanov

Patrick McCauley

Carole Anastasi

Mark Smith

Joseph Brennan

Antoine Francais

Roberto Rigatti

Tobias William Barr Ost

Randall Smith

Anthony Romieu

Gerardo Turcatti

David James Earnshaw

Graham John Worsley

Michael Callingham

Helmy A Eltoukhy

Jonathan Mark Boutell

Robert C Kain

Wenyi Feng

Min-Jui Richard Shen

Bernard Hirschbein

Kay Klausing

Thomas J Joseph

Niall Hynes

Carolyn Tregidgo

Shankar Balasubramanian

Elena Cressina

Adam Culley

Angelica Mariani

Isabelle Rasolonjatovo

Wayne N George

Harold Philip Swerdlow

Isabelle Marie Julia Rasolonjatovo

Natasha Crake

Andrew Antony Brown

John M Beierle

Peyton Shieh

Donald A Wellings

Liu Xiaohai

Jie Mao

Elliot John Lawrence

Patrick Mccauley

Isabelle Marie J Rasolonjatovo

Thomas Joseph

A polynucleotide sequencing method includes a wash step that employs a composition including a polymerase. The composition may also include a plurality of nucleotides. The composition may be configured to prevent the polymerase from incorporating one of the plurality of nucleotides into a copy polynucleotide strand. The composition may be substantially free of Mg.

Polynucleotide sequencing

The present application relates to substituted coumarin derivatives and their uses as fluorescent labels. These compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Substituted coumarin dyes and uses as fluorescent labels

Embodiments of the present disclosure relate to nucleotide molecules with a 3' AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3′-OH blocking group for sequencing applications.

Nucleotides with a 3' AOM blocking group

Embodiments of the present disclosure relate to cyclooctatetraene containing dyes and their uses as fluorescent labels. Also provided are composition containing cyclooctatetraene. The dyes and compositions may be used in various biological applications, such as nucleic acid sequencing.

Cyclooctatetraene containing dyes and compositions

Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by NaSO. Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides

Described herein are heterocyclic azide-containing monomer units, copolymers comprising such heterocyclic azide-containing monomer units, substrate-bound copolymers, and oligonucleotide-bound copolymers, methods for making such copolymers and reacting them with a substrate and/or oligonucleotide, and methods of using such copolymers for immobilization of oligonucleotides to a substrate, for example for use in DNA sequencing or other diagnostic applications.

Heterocyclic azide units and their use in polymer coatings

The present application relates to secondary amine-substituted coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

Secondary amine-substituted coumarin compounds and their uses as fluorescent labels

The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Methods and compositions for nucleic acid sequencing

Embodiments of the present disclosure relate to methods of preparing growing polynucleotides using nucleotide molecules with a 3' AOM blocking group. Also provided herein are kits related to such methods.

Methods of preparing growing polynucleotides using nucleotides with 3' AOM blocking group

Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.

Modified nucleotide linkers

Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3' AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.

Sequencing methods using nucleotides with 3' AOM blocking group

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Cambridge, United Kingdom

Xiaolin Wu