Tianjin University of Science and Technology

Xiaole Wu

Dongguang Xiao

Xuewu Guo

Yefu Chen

Guo Zhang

Huan Wang

Peng Zheng

Yanrui Ma

Jinying Ren

Yongjing Du

Yanfang Chen

Xinyue Kang

Ruirui Li

Wenqi Shi

Sen Jiang

Chunhong Sun

The present disclosure belongs to the field of bioengineering, and relates to breeding of industrial microorganisms, in particular to a genetically engineered strain of, method for constructing the same, and its use for brewing, the genetically engineered strain ofheterogeneously overexpresses an acetaldehyde dehydrogenase gene ALD6, an acetyl-CoA synthase gene ACS1 and an alcohol acyltransferase gene AeAT9. Thestrain with high yield of ethyl acetate and low yield of higher alcohols provided by the present disclosure not only maintains excellent ethanol fermentation characteristics, but also reducing the production of higher alcohols which adversely affect the comfort after drinking, which is of great significance for a well-maintained and strengthened flavor characteristics of Chinese Baijiu, an improved and stabilized quality thereof, and even a reform in the fermentation process thereof.

Genetically engineered strain of, method for constructing the same and its use for brewing

Astrain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33&#xb1;3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65&#xb1;7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93&#xb1;3.18 mg/L of ethyl crotonate is unexpectedly produced.

strain with high yield of ethyl butyrate and construction method and application ofstrain

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Tianjin, China

Xiaole Wu